FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003293718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4733609
Sequences flagged as poor quality0
Sequence length126
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1441563.045371935028854RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1228462.5951868859468536RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGTATGC1222672.582955203946925RNA PCR Primer, Index 14 (100% over 50bp)
CCCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCAT1059362.2379541698522205RNA PCR Primer, Index 14 (100% over 41bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC941331.9886095366136072No Hit
CCCCGGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCAT922941.9497596865309323RNA PCR Primer, Index 14 (100% over 41bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA741741.566965078864773RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC622081.314176984199582RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA599251.2659473986972731RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT553571.169445976632206RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA469710.9922872801703733RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA467430.9874706592792095RNA PCR Primer, Index 1 (100% over 28bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCG339990.718246902099434RNA PCR Primer, Index 14 (100% over 45bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA318230.6722777483311358RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCGGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCA312020.6591587940617825RNA PCR Primer, Index 14 (100% over 40bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTC288570.6096194256855605RNA PCR Primer, Index 14 (100% over 44bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA284690.6014227199584925No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG264680.5591505339794647RNA PCR Primer, Index 1 (100% over 29bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGTAT246670.5211034540453172RNA PCR Primer, Index 14 (100% over 48bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA246590.520934449803522RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA230680.4873237312164989RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC211400.4465937089438523RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC203450.429798912415453RNA PCR Primer, Index 1 (100% over 27bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA199570.42160220668838516RNA PCR Primer, Index 1 (100% over 32bp)
CCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCT195910.4138702626262541RNA PCR Primer, Index 14 (100% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCT193880.4095817799907005RNA PCR Primer, Index 14 (100% over 43bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC192990.40770160780072884RNA PCR Primer, Index 1 (100% over 26bp)
CCCCGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCA190040.40146957638453024RNA PCR Primer, Index 14 (100% over 40bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA189960.4013005721427351RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA189870.4011104423707154RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC185160.39116031763502224RNA PCR Primer, Index 1 (100% over 26bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG179900.38004828873698693RNA PCR Primer, Index 1 (100% over 29bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGTATGCC173530.36659132598404304RNA PCR Primer, Index 14 (98% over 50bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC164260.34700795946602264No Hit
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA161980.34219133857485906RNA PCR Primer, Index 1 (100% over 34bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGTATGCC154000.3253331654557865RNA PCR Primer, Index 14 (100% over 50bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTC152710.32260797205683867RNA PCR Primer, Index 14 (100% over 44bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG149170.31512953435740043RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC144510.30528503727282924No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA131600.2780119777531266RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG126390.2670055765062133RNA PCR Primer, Index 1 (100% over 29bp)
CGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCT119850.2531894797394546RNA PCR Primer, Index 14 (100% over 43bp)
CCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATC115400.24378861878959585RNA PCR Primer, Index 14 (100% over 42bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC112810.2383171064614758RNA PCR Primer, Index 1 (100% over 26bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA111060.2346201386722055RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT108120.22840923278623138RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT105660.22321235235102857RNA PCR Primer, Index 1 (100% over 30bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG104940.22169131417487165RNA PCR Primer, Index 2 (97% over 35bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG103460.21856473570166018RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG102580.21670568904191284RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA102380.21628317843742478RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC100750.2128397170108473RNA PCR Primer, Index 1 (100% over 33bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATC99650.21051590868616313RNA PCR Primer, Index 14 (100% over 42bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG97820.2066499366550976RNA PCR Primer, Index 1 (100% over 29bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG95230.20117842432697758RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT95070.20084041584338716RNA PCR Primer, Index 8 (97% over 36bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA93340.19718569911456565RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAAG91360.1930028441301341No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC91190.19264371011631928RNA PCR Primer, Index 1 (100% over 26bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG90490.19116492300061116RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGC90110.19036215285208388No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC88760.18751020627178966RNA PCR Primer, Index 1 (100% over 27bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT88620.18721444884864805RNA PCR Primer, Index 8 (97% over 36bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA87760.18539765324934948RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA85960.18159505780895718RNA PCR Primer, Index 1 (100% over 28bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG83640.176693934796896RNA PCR Primer, Index 2 (97% over 35bp)
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG81660.17251107981246444RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA79970.16894086520454057RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA76500.16161030621667316RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA76380.16135679985398033No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA74130.15660355555348995RNA PCR Primer, Index 1 (100% over 28bp)
CGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCT73940.15620217047922633RNA PCR Primer, Index 14 (100% over 43bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC73210.154660006772845No Hit
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG72980.15417411957768376RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT72730.15364598132207372RNA PCR Primer, Index 1 (100% over 30bp)
CCGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATC71550.15115316875559429RNA PCR Primer, Index 14 (100% over 42bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTC69690.1472238201338556RNA PCR Primer, Index 14 (100% over 44bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGT68770.14528027135321062RNA PCR Primer, Index 14 (100% over 46bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA67200.14196356310797956RNA PCR Primer, Index 1 (100% over 34bp)
CCCGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCAT66750.14101291424788148RNA PCR Primer, Index 14 (100% over 41bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA66030.13949187607172456No Hit
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG65750.1389003612254413RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA64660.1365976784309815No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC64360.13596391252424947No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC63190.13349222548799447RNA PCR Primer, Index 1 (100% over 26bp)
CCCCGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATC62950.13298521276260883RNA PCR Primer, Index 14 (100% over 42bp)
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCC62470.13197118731183755RNA PCR Primer, Index 14 (100% over 39bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC62400.13182330860026673RNA PCR Primer, Index 1 (100% over 27bp)
CCGTCGTGAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTC62030.13104166398196387RNA PCR Primer, Index 14 (100% over 38bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA61820.13059802784725144No Hit
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT59990.1267320558161859RNA PCR Primer, Index 1 (100% over 30bp)
AACCCGTAGATCCGATCTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG59580.12586590907698544RNA PCR Primer, Index 1 (100% over 29bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCG58340.12324634332915962RNA PCR Primer, Index 14 (100% over 45bp)
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCA58070.12267595401310079RNA PCR Primer, Index 14 (100% over 40bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA57920.12235907105973476No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA57840.12219006681793955No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC57810.12212669022726634No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA57680.12185205833434912No Hit
CGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCT57370.12119716689739267RNA PCR Primer, Index 14 (100% over 43bp)
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA55830.1179438352428348RNA PCR Primer, Index 1 (100% over 28bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTT54440.11500738654164297RNA PCR Primer, Index 8 (97% over 37bp)
CCCCGGGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCA52460.1108245315572114RNA PCR Primer, Index 14 (100% over 40bp)
CGGCGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCA52300.11048652307362099RNA PCR Primer, Index 14 (100% over 40bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCC50360.10638817021008705RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTCGTCCCGGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT49750.10509951286639856RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAAGTTGTATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA49330.10421224059697369RNA PCR Primer, Index 1 (100% over 28bp)
AGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATC49320.10419111506674927RNA PCR Primer, Index 14 (100% over 42bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC49160.10385310658315886RNA PCR Primer, Index 1 (100% over 26bp)
GTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCT48380.10220531522565553RNA PCR Primer, Index 14 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph