FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003293719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003293719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4733609
Sequences flagged as poor quality0
Sequence length126
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT1347762.8472144615239663Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT1073982.268839695040296Illumina DpnII expression Sequencing Primer (96% over 28bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA981412.0732806617530093Illumina RNA PCR Primer (100% over 45bp)
GCCCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG954662.0167698684027346Illumina RNA PCR Primer (100% over 36bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA868851.8354916935471435No Hit
TCCCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG849851.79535318612078Illumina RNA PCR Primer (100% over 36bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT690501.4587178619949388Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG597961.263222205298325Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGT575811.2164291558512754Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT522621.1040624605876828Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT444790.939642458851164Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT439950.9294177022225536Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG315050.6655598297197761Illumina RNA PCR Primer (100% over 40bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT306740.6480045141032983Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCCCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT286790.6058590813056169Illumina RNA PCR Primer (100% over 35bp)
GCCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC262010.5535100174095494Illumina RNA PCR Primer (100% over 39bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA256570.5420177289674749No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG247580.5230258772957378Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT225530.47644408315093195Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT212620.4491710236312294Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG207970.4393476520768826Illumina RNA PCR Primer (100% over 43bp)
GTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACG191840.4052721718249226Illumina DpnII expression Sequencing Primer (96% over 26bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG188250.39768810647436237Illumina DpnII expression Sequencing Primer (96% over 26bp)
CCACGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT181150.38268898001503715Illumina RNA PCR Primer (100% over 38bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC180120.3805130504019238Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT178660.37742872298916114Illumina RNA PCR Primer (100% over 38bp)
CCGCTAAGAGTCGTACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG177680.37535842102716976Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT177250.37445002322752047Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAAC177020.37396413603235923Illumina DpnII expression Sequencing Primer (100% over 26bp)
ATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT175340.37041504695465977Illumina RNA PCR Primer (100% over 46bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT174990.3696756533968057Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG164140.34675445310332986Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCCCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT163820.34607843613614897Illumina RNA PCR Primer (100% over 35bp)
AGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC144900.3061089329515809Illumina RNA PCR Primer (100% over 39bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA144620.3055174181052977No Hit
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG139840.2954194146580337Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCGCTAAGAGTCGTACGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT131690.27820210752514624Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA130420.27551916518664726No Hit
CAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGTG122750.2593158835045311Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT113270.23928888085179828Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG110700.23385961958412704Illumina DpnII expression Sequencing Primer (95% over 24bp)
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT101000.21336785526645738Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC100680.21269183829927654Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG100170.21161443625783202Illumina RNA PCR Primer (100% over 37bp)
GGCCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT99690.21060041080706074Illumina RNA PCR Primer (100% over 38bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT99300.20977651512830908Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG95790.20236145401954408Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG94860.2003967797086747Illumina RNA PCR Primer (100% over 37bp)
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG94600.19984751592284025Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT94470.19957288402992304Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA91390.1930662207208073Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC87930.18575678726316433Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT87400.18463713416127103Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTAGTGCTTTCTACTTTATGGATCGTCGGACTGTAGAACTCTGAACGTG87260.1843413767381294Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT85350.18030640046526866No Hit
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG84760.17905999418202898Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG83570.17654605608532517Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT83210.17578553699724672Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT82360.17398986692817256Illumina DpnII expression Sequencing Primer (96% over 28bp)
GATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT81970.1731659712494209Illumina RNA PCR Primer (100% over 31bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG81400.17196181602663No Hit
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG81210.17156043095236637Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT81030.17118017140832714Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTG78950.16678606112165156Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT78130.16505376764325064Illumina RNA PCR Primer (100% over 31bp)
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT75590.15968788296625258Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC75010.1584626022132373Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT73400.15506139184710863Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC69490.14680130952936754No Hit
ACAAAGTTCTGTAGTGCACTGAGATCGTCGGACTGTAGAACTCTGAACGT69110.1459985393808403Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA69070.14591403725994267No Hit
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC67360.14230157159157Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACAGCCCATCGACTGGTGTTGGATCGTCGGACTGTAGAACTCTGAACGTG66950.1414354248523695Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT66500.14048477599227144Illumina RNA PCR Primer (100% over 38bp)
ACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT64000.135203393436171Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC62450.13192893625138874Illumina RNA PCR Primer (100% over 39bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG62350.1317176809491447Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG60880.12861222800615768Illumina RNA PCR Primer (100% over 37bp)
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT60810.12846434929458686Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG60420.1276404536158352Illumina DpnII expression Sequencing Primer (96% over 26bp)
TTACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAAC59510.12571803036541465Illumina DpnII expression Sequencing Primer (100% over 26bp)
CCCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG59370.125422272942273Illumina RNA PCR Primer (100% over 37bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA59220.12510538998890697No Hit
GCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGC58750.1241124900683601Illumina RNA PCR Primer (100% over 41bp)
TGGGCCCGATAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG57830.12216894128771515Illumina RNA PCR Primer (100% over 34bp)
AGCCCCGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG57570.12161967750188071Illumina RNA PCR Primer (100% over 36bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA57550.1215774264414319No Hit
GTCTCACGACGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG57350.12115491583694386Illumina RNA PCR Primer (100% over 33bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA56560.11948599894921613No Hit
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA55700.1176692033499176No Hit
AGTGCTTTCTACTTTATGGGGATCGTCGGACTGTAGAACTCTGAACGTGT54290.11469050358827694Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA53400.1128103313983052No Hit
GCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG53200.11238782079381715Illumina RNA PCR Primer (100% over 40bp)
GCCCGCCGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT52840.11162730170573869Illumina RNA PCR Primer (100% over 35bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC52630.11118366557102626No Hit
GCCGCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT52040.10993725928778654Illumina RNA PCR Primer (100% over 38bp)
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT52040.10993725928778654Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAGATCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG51710.10924011679038129Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA51610.10902886148813729No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA50860.10744444672130715No Hit
TCCCCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT48050.10150817272825026Illumina RNA PCR Primer (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)