FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003294862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003294862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21713396
Sequences flagged as poor quality0
Sequence length35-69
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21513679.908017152176471No Hit
ACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG694280.3197473117516947No Hit
TTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG518280.23869135901173635No Hit
GTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG425380.195906711230247No Hit
GTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG421230.19399544870825364No Hit
AGGTGGGGGGGTTAGTTTTTCGTTTGNNNNNNNNN341900.15746039910108947No Hit
CGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAGGCGTTCGTTATTACGTTTG288650.13293636794539188No Hit
GGGTTGATTTTTTTATTTTTTTTTTGNNNNNNNNN279530.12873619584886675No Hit
TTTATTGGGAAGTGAGGAGTTTTTTTGTTTGNNNN251250.11571197798815074No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)