FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003294863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003294863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26321952
Sequences flagged as poor quality0
Sequence length35-69
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN430702516.3628632101449No Hit
TTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG584010.22187184293930787No Hit
GTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG502430.19087870078936395No Hit
GAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG494160.18773683653856674TruSeq Adapter, Index 7 (100% over 55bp)
GTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG494020.1876836489938132No Hit
ACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG438320.16652260440259142No Hit
CGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAGGCGTTCGTTATTACGTTTG307630.1168720313751807No Hit
AGGTGGGGGGGTTAGTTTTTCGTTTGNNNNNNNNN293720.11158746889288454No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)