FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003294867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003294867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23158545
Sequences flagged as poor quality0
Sequence length35-69
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN369627415.960735011633934No Hit
GAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG3159651.3643560076852843TruSeq Adapter, Index 1 (100% over 55bp)
GAGCACACGTCTGAACTCCAGTCAACACACTATCCCTAAACCACGCTAATCCGATAAAAAAAAAAGAGC417320.1802013036656664TruSeq Adapter, Index 7 (96% over 27bp)
AGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG393400.1698725027846093TruSeq Adapter, Index 1 (100% over 56bp)
TTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG293300.12664871648888132No Hit
ACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG232280.10029991089682015No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)