FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003294873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003294873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33397927
Sequences flagged as poor quality0
Sequence length35-69
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32709729.793937210534056No Hit
GAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG3026210.9061071365297612TruSeq Adapter, Index 9 (100% over 55bp)
TTTATTGGGAAGTGAGGAGTTTTTTTGTTTGNNNN1015640.3041027067338641No Hit
AGGTGGGGGGGTTAGTTTTTCGTTTGNNNNNNNNN826950.247605188190273No Hit
ACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG687840.20595290240618827No Hit
GGGTTGATTTTTTTATTTTTTTTTTGNNNNNNNNN649850.19457794491256897No Hit
TTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG574300.17195678043131238No Hit
GGGGTTTTTTATTTTTTAGTAGGGGCGGTTGNNNN531500.1591416137893828No Hit
GTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG483730.14483833083412634No Hit
GTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG481600.14420056669984338No Hit
GGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTG363300.10877920656572487No Hit
GGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTTTTG342560.10256924030045338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)