FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003294875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003294875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32457779
Sequences flagged as poor quality0
Sequence length35-69
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27045968.332658867385843No Hit
GAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG897690.27657160399052566TruSeq Adapter, Index 11 (100% over 55bp)
TTTATTGGGAAGTGAGGAGTTTTTTTGTTTGNNNN868870.26769237661024187No Hit
AGGTGGGGGGGTTAGTTTTTCGTTTGNNNNNNNNN710880.21901683414629203No Hit
ACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG679480.20934272797901543No Hit
GGGTTGATTTTTTTATTTTTTTTTTGNNNNNNNNN621790.19156886859079297No Hit
TTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG587240.18092427088125776No Hit
GTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG498180.15348554810235168No Hit
GGGGTTTTTTATTTTTTAGTAGGGGCGGTTGNNNN490660.15116869210305486No Hit
GTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG490570.15114096377327604No Hit
CGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAGGCGTTCGTTATTACGTTTG381780.11762357492174681No Hit
GGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTG377070.1161724589966553No Hit
GGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTTTTG333300.10268724794755674No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)