FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003294878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003294878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15713473
Sequences flagged as poor quality0
Sequence length35-69
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14907659.48717702318259No Hit
ACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG220660.14042726264270158No Hit
TTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG215050.13685707799924307No Hit
AGGTGGGGGGGTTAGTTTTTCGTTTGNNNNNNNNN204100.1298885357807278No Hit
GGGTTGATTTTTTTATTTTTTTTTTGNNNNNNNNN189770.1207689732244425No Hit
TTTATTGGGAAGTGAGGAGTTTTTTTGTTTGNNNN183920.11704605340907132No Hit
GTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG178720.1137367913509636No Hit
GTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG173520.11042752929285589No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)