FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003294881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003294881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19458636
Sequences flagged as poor quality0
Sequence length35-69
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15544847.988658608959025No Hit
AGGTGGGGGGGTTAGTTTTTCGTTTGNNNNNNNNN397720.2043925380997928No Hit
TTTATTGGGAAGTGAGGAGTTTTTTTGTTTGNNNN385260.19798921157680324No Hit
GGGTTGATTTTTTTATTTTTTTTTTGNNNNNNNNN290260.14916770116877667No Hit
GTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG220960.11355369410271102No Hit
GGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTG217900.11198112755693666No Hit
GTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG212010.10895419391163902No Hit
TTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG202020.10382022665925814No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)