FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003294884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003294884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22202413
Sequences flagged as poor quality0
Sequence length35-69
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17135157.7176971710237074No Hit
TTTATTGGGAAGTGAGGAGTTTTTTTGTTTGNNNN516600.23267741213533863No Hit
AGGTGGGGGGGTTAGTTTTTCGTTTGNNNNNNNNN435840.1963029874275377No Hit
GGGTTGATTTTTTTATTTTTTTTTTGNNNNNNNNN349250.15730272200593692No Hit
GTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG333680.1502899707342621No Hit
GTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG321950.1450067612020369No Hit
ACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG310160.13969652758013285No Hit
TTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG277710.12508099907879383No Hit
GGGGTTTTTTATTTTTTAGTAGGGGCGGTTGNNNN223460.10064671799412074No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)