FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003294889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003294889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21527738
Sequences flagged as poor quality0
Sequence length35-69
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20963719.737999412664722No Hit
ACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG390280.1812916898189675No Hit
AGGTGGGGGGGTTAGTTTTTCGTTTGNNNNNNNNN364750.16943257113218305No Hit
TAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATAAGAATATAAATTTTAGGGTGATTGAA294470.13678631726194362No Hit
TTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG258100.11989183443239601No Hit
CGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAGGCGTTCGTTATTACGTTTG247820.11511659980254312No Hit
GGGTTGATTTTTTTATTTTTTTTTTGNNNNNNNNN241240.1120600780258474No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)