FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003294893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003294893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21728307
Sequences flagged as poor quality0
Sequence length35-69
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN261945412.055490563530789No Hit
AGGTGGGGGGGTTAGTTTTTCGTTTGNNNNNNNNN275010.12656761523113605No Hit
ACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG270560.1245195955672018No Hit
TTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG267660.12318493106710984No Hit
CGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAGGCGTTCGTTATTACGTTTG257470.1184951961512694No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)