FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003294898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003294898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20583389
Sequences flagged as poor quality0
Sequence length35-69
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN259217512.593528694424421No Hit
AGGTGGGGGGGTTAGTTTTTCGTTTGNNNNNNNNN420700.2043881112094806No Hit
GGGTTGATTTTTTTATTTTTTTTTTGNNNNNNNNN322840.15684491994977115No Hit
TTTATTGGGAAGTGAGGAGTTTTTTTGTTTGNNNN249580.12125311337214684No Hit
GGGGTTTTTTATTTTTTAGTAGGGGCGGTTGNNNN246760.11988307659151756No Hit
CGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAGGCGTTCGTTATTACGTTTG225780.10969039160655225No Hit
GGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTG215480.10468635655673611No Hit
TTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG212170.10307826373975636No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)