FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003294899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003294899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27135221
Sequences flagged as poor quality0
Sequence length35-69
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN430690315.87200266399157No Hit
ACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG447270.16483005611046986No Hit
AGGTGGGGGGGTTAGTTTTTCGTTTGNNNNNNNNN396170.145998442393375No Hit
TTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG324710.1196636651678643No Hit
GGGTTGATTTTTTTATTTTTTTTTTGNNNNNNNNN319150.1176146676675307No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)