FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003294903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003294903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23753426
Sequences flagged as poor quality0
Sequence length35-69
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN283463511.933583812288804No Hit
TAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATAAGAATATAAATTTTAGGGTGATTGAA563890.2373931238382202No Hit
ACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG438620.18465546822593085No Hit
GTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAATATTAAGTTGAGATGATATTATTTATG350640.14761660065373305No Hit
TTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG328300.13821164155435936No Hit
GAGATTTTATTTTATATTAATATTTATTTTGATTTTTTGGTTATTTTGAAGTTTATATTTTTATTTTAT327850.13802219519828424No Hit
AGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAGGGGGTTATTTTTAGTTTTATTGTTAT307920.1296318265836684No Hit
TAAATTAAATTATTTTTATTGTTATATGATTGNNN295560.12442836667013846No Hit
CGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAGGCGTTCGTTATTACGTTTG289370.1218224267943496No Hit
AGGTGGGGGGGTTAGTTTTTCGTTTGNNNNNNNNN284490.11976798631069051No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)