FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003294905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003294905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21383209
Sequences flagged as poor quality0
Sequence length35-69
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14510216.78579627594717No Hit
TTTATTGGGAAGTGAGGAGTTTTTTTGTTTGNNNN559920.26185031442193735No Hit
AGGTGGGGGGGTTAGTTTTTCGTTTGNNNNNNNNN476980.22306287143337558No Hit
TAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATAAGAATATAAATTTTAGGGTGATTGAA474560.22193114232760855No Hit
GTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG374450.17511403456796404No Hit
GTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG357290.16708904636343402No Hit
GGGTTGATTTTTTTATTTTTTTTTTGNNNNNNNNN354700.16587781562627013No Hit
GAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG350330.16383415604271556TruSeq Adapter, Index 2 (100% over 55bp)
AGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAGGGGGTTATTTTTAGTTTTATTGTTAT348710.16307655226116904No Hit
ACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG341930.15990584013840017No Hit
GGGGTTTTTTATTTTTTAGTAGGGGCGGTTGNNNN326180.1525402478178088No Hit
GAGATTTTATTTTATATTAATATTTATTTTGATTTTTTGGTTATTTTGAAGTTTATATTTTTATTTTAT308890.144454464248093No Hit
CGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAGGCGTTCGTTATTACGTTTG250210.11701237171651832No Hit
TTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG243660.11394922062446287No Hit
GGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTG243530.11388842525927705No Hit
GTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAATATTAAGTTGAGATGATATTATTTATG239540.11202247520472723No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)