FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003294906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003294906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22327506
Sequences flagged as poor quality0
Sequence length35-69
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20896079.35889122591659No Hit
TTTATTGGGAAGTGAGGAGTTTTTTTGTTTGNNNN695860.31166042459018933No Hit
AGGTGGGGGGGTTAGTTTTTCGTTTGNNNNNNNNN584660.2618563846763718No Hit
GAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG503850.22566335890806616TruSeq Adapter, Index 3 (100% over 55bp)
GGGTTGATTTTTTTATTTTTTTTTTGNNNNNNNNN452980.20287980215972176No Hit
GGGGTTTTTTATTTTTTAGTAGGGGCGGTTGNNNN363930.16299626120355762No Hit
ACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG327920.14686817237894817No Hit
GTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG318600.14269394888976852No Hit
GTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG305780.13695215220186258No Hit
TTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG265620.11896536944162056No Hit
GGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTG243950.10925985195122105No Hit
CGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAGGCGTTCGTTATTACGTTTG224730.10065163569992995No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)