FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003294907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003294907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28414435
Sequences flagged as poor quality0
Sequence length35-69
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25256888.888749679520286No Hit
TTTATTGGGAAGTGAGGAGTTTTTTTGTTTGNNNN588430.20708840418611174No Hit
TAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATAAGAATATAAATTTTAGGGTGATTGAA502790.1769487938084991No Hit
AGGTGGGGGGGTTAGTTTTTCGTTTGNNNNNNNNN499180.1756783128012223No Hit
GTATTTGTTTTTAAGTTTAATGTGGGGATAGTTTATGAGTGTAAGATGTTTTGTGATGTAATTATTAT388610.13676499286366242No Hit
GGGTTGATTTTTTTATTTTTTTTTTGNNNNNNNNN376360.13245380385005015No Hit
AGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAGGGGGTTATTTTTAGTTTTATTGTTAT368150.12956442737643736No Hit
AAATGTGTGGGGGTAGTAGGGTGATTTGNNNNNNN361870.12735428313109165No Hit
GGGGTTTTTTATTTTTTAGTAGGGGCGGTTGNNNN343580.12091741398342075No Hit
TGGAAGATATGTAGAGTTTGGTTGGTGGAATGGTGGTTTG337290.11870375040010474No Hit
GAGATTTTATTTTATATTAATATTTATTTTGATTTTTTGGTTATTTTGAAGTTTATATTTTTATTTTAT325860.11468114710005671No Hit
TGAAAAATAGGTTGAGTATGGTGTATGTTAGTTTG322760.11359015232926505No Hit
ACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG321400.11311152236530482No Hit
GTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG317280.11166155512154297No Hit
GTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG304740.10724830530679214No Hit
GGTAGTGTGATTTTATGGTTGAGTGTTAGTGTTTG291810.10269780131119974No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)