FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003294908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003294908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25606844
Sequences flagged as poor quality0
Sequence length35-69
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23538609.192308118876344No Hit
TTTATTGGGAAGTGAGGAGTTTTTTTGTTTGNNNN697900.2725443244782528No Hit
AGGTGGGGGGGTTAGTTTTTCGTTTGNNNNNNNNN576760.22523665938684206No Hit
GGGTTGATTTTTTTATTTTTTTTTTGNNNNNNNNN422870.1651394447515672No Hit
TAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATAAGAATATAAATTTTAGGGTGATTGAA417010.16285099405455822No Hit
GTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG388800.1518344080199809No Hit
ACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG376250.14693337453065283No Hit
GGGGTTTTTTATTTTTTAGTAGGGGCGGTTGNNNN372370.14541815461522709No Hit
GTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG370600.1447269331589633No Hit
GGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTG293750.11471542529801798No Hit
TTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG289440.1130322815259858No Hit
AGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAGGGGGTTATTTTTAGTTTTATTGTTAT279060.10897867773162519No Hit
CGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAGGCGTTCGTTATTACGTTTG267220.10435491386599613No Hit
GGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTTTTG265000.10348795814119069No Hit
GAGATTTTATTTTATATTAATATTTATTTTGATTTTTTGGTTATTTTGAAGTTTATATTTTTATTTTAT263520.10290998765798706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)