FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003294910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003294910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26914445
Sequences flagged as poor quality0
Sequence length35-69
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN361036813.41423908239609No Hit
GTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG458450.17033604073946165No Hit
GAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG415810.15449324702775777TruSeq Adapter, Index 7 (100% over 55bp)
GTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG407470.15139453925206334No Hit
ACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG344630.12804648210282618No Hit
CGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAGGCGTTCGTTATTACGTTTG305560.1135301136620131No Hit
TTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG281990.10477273449257453No Hit
GGGTTGATTTTTTTATTTTTTTTTTGNNNNNNNNN271890.10102010277380791No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)