FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003294914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003294914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22682719
Sequences flagged as poor quality0
Sequence length35-69
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN313349313.814450551541022No Hit
TGATTTTTAATATGGAAGTTAAATGTTTGNNNNNN440290.1941081225756048No Hit
ACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG358570.15808069570495495No Hit
TTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG338390.1491840550508958No Hit
AGGTGGGGGGGTTAGTTTTTCGTTTGNNNNNNNNN294150.1296802204356541No Hit
CGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAGGCGTTCGTTATTACGTTTG264580.11664386443265466No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)