FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003294915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003294915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25598127
Sequences flagged as poor quality0
Sequence length35-69
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN265316510.364684103645553No Hit
GAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG4159891.6250759284067933TruSeq Adapter, Index 3 (100% over 55bp)
ACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG683570.2670390689131279No Hit
TTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG517940.20233511615908462No Hit
TTTATTGGGAAGTGAGGAGTTTTTTTGTTTGNNNN457980.17891152739417224No Hit
GTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG391840.15307369949371685No Hit
GTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTG382560.14944843425458434No Hit
AGGTGGGGGGGTTAGTTTTTCGTTTGNNNNNNNNN364980.14258074428648626No Hit
GGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTG321540.12561075269296068No Hit
GGGTTGATTTTTTTATTTTTTTTTTGNNNNNNNNN310650.12135653518712522No Hit
GGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTTTTG306470.11972360321518835No Hit
CGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAGGCGTTCGTTATTACGTTTG303350.11850476403996277No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)