FastQCFastQC Report
Fri 3 May 2024
EGAF00003435195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003435195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31121728
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG837290.2690371177333084No Hit
GTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCA469640.15090421714372673No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA431200.13855271789535592No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATT358620.1152313907505393No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG325060.10444792782714378No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT322210.10353216890784471TruSeq Adapter, Index 15 (97% over 40bp)
CTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCC319490.10265818144802243No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)