FastQCFastQC Report
Wed 15 May 2024
EGAF00003435269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003435269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37610728
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT1426750.37934655239856035TruSeq Adapter, Index 18 (97% over 40bp)
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG688690.18310998925625688No Hit
CTTGGATTAAGGCGACAGCGATTTCTAGGATAGTCAGTAGAATTAGAATT447050.11886236288752507No Hit
GTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCA423040.1124785460148498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)