FastQCFastQC Report
Sun 12 May 2024
EGAF00003435315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003435315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28219597
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG838670.2971941803421218No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT470090.1665828183159384TruSeq Adapter, Index 16 (97% over 40bp)
GTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCA449990.15946010851962203No Hit
GTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTG378010.13395301144803734No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA350270.12412296320177783No Hit
GGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGAGCTACAACGT329450.11674511156201133No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATT291120.10316235203500602No Hit
CTTGGATTAAGGCGACAGCGATTTCTAGGATAGTCAGTAGAATTAGAATT289030.10242173196165771No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG286530.1015358227830114No Hit
CTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCC286150.10140116458785715No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)