FastQCFastQC Report
Thu 23 Mar 2023
EGAF00003442363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003442363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53283394
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT6613331.24116155213386No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT4875610.9150336782225247No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG2997020.562467923871366No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG2832930.5316722129224726No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA2761930.5183472359136883No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT2705740.5078017365034967No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC2425990.4552994503315611No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT2287750.42935515706826033No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA2280570.42800764530878044No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG2168620.4069973470533803No Hit
CGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT2138270.40130138857145625No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT2056430.38594200662217576No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC2026280.3802835832867554No Hit
GTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAG1896750.3559739456536871No Hit
CCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTC1567840.2942455204711622No Hit
CAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCT1545640.2900791192092606No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT1466410.27520957092185233No Hit
GTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAG1460630.2741248051878978No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT1447710.27170003472376403No Hit
TGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCA1241580.23301443597981017No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT1241300.23296188677470508No Hit
ATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGT1198220.22487681621782576No Hit
CAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCT1166350.2188955906224742No Hit
CCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTC1154380.2166491121042327No Hit
TGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCA1075810.20190342980028636No Hit
CTTAGCTCTCACCATCGCTCTTCTACTATGAACCCCCCTCCCCATACCCA1013600.190228122480336No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG1001800.18801354883662252No Hit
CCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAA989310.1856694789374716No Hit
CCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTT953130.17887937093496709No Hit
CTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC919190.1725096565733031No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC874390.1641017837564927No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT845850.1587455183504264No Hit
CAGAGACCCAGCCCGAGTTTCCCATCGCACTGAGCACTGAGATCCTGCTG808970.15182403733515923No Hit
GTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAG764170.14341616451834882No Hit
CTTAATTCCATCCACCCTCCTCTCCCTAGGAGGCCTGCCCCCGCTAACCG763440.1432791612336106No Hit
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA760150.14266170807362608No Hit
CTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC710200.13328730523434748No Hit
CCTCAACCTAGGCCTCCTATTTATTCTAGCCACCTCTAGCCTAGCCGTTT700790.13152127659135227No Hit
CTCTGATTACTCCTGCCATCATGACCCTTGGCCATAATATGATTTATCTC697450.13089443964474184No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG674310.12655162319427327No Hit
CTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG673830.12646153884266456No Hit
GTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAG606730.11386849719070072No Hit
CACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTT600070.11261857681213025No Hit
CTTAATATGACTAGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTT573040.10754570176216627No Hit
CCCTCCTCTCCCTAGGAGGCCTGCCCCCGCTAACCGGCTTTTTGCCCAAA566710.10635771437532678No Hit
CGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTA560400.10517348050313761No Hit
CTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTG560290.10515283617256062No Hit
CTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTG554700.1041037288277845No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT550210.10326106478877829No Hit
CCCAGCCCGAGTTTCCCATCGCACTGAGCACTGAGATCCTGCTGGAAGCT543440.10199050007963081No Hit
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT535810.10055853424051778No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)