FastQCFastQC Report
Thu 16 May 2024
EGAF00003442962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003442962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80292241
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA17837992.2216330965279694No Hit
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC16068832.0012930016488144No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA10251271.276744785339844No Hit
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT6133210.7638608567420605No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT5360080.6675713535010188No Hit
ACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGT4920030.6127653106605905No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC3999770.49815149635691447No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG3901100.4858626377111582No Hit
CTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCA3465780.4316456928883079No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTCC3361810.4186967455547791No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCC3218510.40084944197783695No Hit
CTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGG3139530.39101287507967303No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2610540.3251297967882102No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2555660.31829476524387956TruSeq Adapter, Index 12 (100% over 50bp)
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA1963920.2445964859792617No Hit
CAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGTATTG1932120.24063595385262695No Hit
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC1795820.22366046552368615No Hit
CCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGAT1715550.21366323552981914No Hit
CACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAG1622510.20207556543352678No Hit
GCCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGT1573190.19593300428618005No Hit
CGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTCCAA1529470.1904878953372344No Hit
CCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGATA1507320.18772922280248722No Hit
CCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCA1391290.17327826234168753No Hit
CAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGGATTG1346840.1677422355168789No Hit
ACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAG1296920.1615249473482749No Hit
CGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCCAA1292830.16101555815337126No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG1276500.15898173772481952No Hit
TGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCAT1246090.15519432319743073No Hit
GTCAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGTAT1233950.15368234646732554No Hit
CTCCTATTCCATCTCCCTGCTCCAAAAATCCATTTAATATATTGTCCTCG1211070.15083275605671537No Hit
ATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCC1194760.14880142652887218No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1180930.14707896868889236No Hit
CGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGC1121250.13964612097450363No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1066170.1327861804230872No Hit
GTCAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGGAT1053420.13119823122137045No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1043500.1299627444699171No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC1016000.12653775599562603No Hit
CTCCTATTTCCAAAAATCCATTTAATATATTGTCCTCGGATAGAGGACGT1014010.1262899113751228No Hit
AAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCA991440.12347892992549554No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG958600.11938887096201488No Hit
CAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGC914790.11393255295988065No Hit
CCTGGAAGTACTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGC912540.11365232663016593No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC905010.11271450251338733No Hit
ACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTA890950.11096339931525886No Hit
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG882310.10988733020915434No Hit
ACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCT882170.10986989390419431No Hit
CCTGGAGGTACTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGC870160.10837410802869483No Hit
CTGAGAATGAGCCCCGTGTGGTTGGTGCGCGGACACGCACTGCCTGCGTA810650.10096243297032899No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)