FastQCFastQC Report
Sun 5 May 2024
EGAF00003442967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003442967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86973589
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2914340.33508333202163243No Hit
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC2536940.2916908488161849No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2500450.2874953222868611No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1708410.19642859627190964No Hit
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA1545610.1777102701832852No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1327740.15266013686062788TruSeq Adapter, Index 10 (100% over 50bp)
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1308310.1504261253378885No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG1118580.1286114569791986No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1036340.11915571289118586No Hit
GTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCACCGCCGCCGCCGCCGCC1018500.11710451548687958No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1002790.11529822001481392No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC957820.11012768485384684No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC943530.10848465733660825No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA926110.10648175045415223No Hit
ACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGT909720.10459727032766233No Hit
CCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG875310.10064089685893036No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG873640.1004488845458591No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)