FastQCFastQC Report
Mon 13 May 2024
EGAF00003442971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003442971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131331405
Total Bases6.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA41052893.1259004653152074No Hit
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC28499492.1700437911252073No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC21333011.6243647130707237TruSeq Adapter, Index 2 (100% over 50bp)
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT15306611.1654950314435455No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA14536091.106825134475642No Hit
ACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGT12048970.9174477346069663No Hit
CAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGGATTG10418070.7932657082287363No Hit
CTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGG10158320.7734874990486852No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTCC8773600.6680504179483955No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC7335720.5585655616796302No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCC6408640.48797467749621654No Hit
CTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCA5409520.4118984335848687No Hit
CACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAG5169910.39365374945924014No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG5030670.3830515633332332No Hit
GTCAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGGAT4884670.3719346488374201No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT4839400.36848764391121835No Hit
CCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGATA4828380.3676485452965344No Hit
CCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGAT4354630.3315756806226203No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA4212940.32078694353418363No Hit
GCCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGT3703400.28198891194379594No Hit
TACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGG3629400.27635431144591804No Hit
ATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCC3520100.2680318542240525No Hit
ACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAG3487380.2655404470849908No Hit
CAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGTATTG3437460.2617393760464224No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC2966930.225911692637416No Hit
CCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCA2961700.22551346344006598No Hit
GCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGG2959800.2253687912651205No Hit
TCAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGGATT2943360.22411699623559192No Hit
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC2645780.20145828790912576No Hit
TGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCAT2619910.19948846203236767No Hit
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAA2431020.18510576354528455No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2289430.17432464078184498No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC2042100.15549213076643778No Hit
CGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGC2004040.15259411867252923No Hit
CGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTCCAA1995140.15191644374778446No Hit
CAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGC1976590.15050398646081642No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG1923290.14644555123734493No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1842290.14027794798966783No Hit
GGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTC1811090.13790227859056253No Hit
CTCCTATTTCCAAAAATCCATTTAATATATTGTCCTCGGATAGAGGACGT1800540.1370989673033651No Hit
CCTGGAAGTACTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGC1789670.1362712901761768No Hit
CACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGATAA1774480.13511467420911244No Hit
TCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGA1680470.12795644727930838No Hit
ACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGG1654500.12597900707755316No Hit
ACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTA1645670.1253066621803064No Hit
AAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCA1643870.125169604330358No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1641580.12499523628792368No Hit
GGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTC1622240.12352262583347828No Hit
GCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCA1552190.11818879117298714No Hit
TTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGG1551280.11811950081551326No Hit
TCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAAT1544260.11758497520071456No Hit
ACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCT1457510.11097954826570233No Hit
CAAAAATTGCCAGTGCCGACTATATTGCAAGTCGTCACGGCGGGGGATTG1453670.11068715818581246No Hit
TTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATG1407640.10718228438963247No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1405910.10705055656718208No Hit
GTCAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGTAT1357160.10333857313108011No Hit
CCGCAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC1340220.1020487064765659No Hit
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG1338280.10190098857162154No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)