FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003443026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003443026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466172
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC272195.838832019083085RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT26960.5783273126657114No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG9870.21172442789356719No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)