FastQCFastQC Report
Thu 16 May 2024
EGAF00003443083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003443083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90890564
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA13243901.457125956441419No Hit
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC12321951.3556907843590893No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA5374970.5913672182736153No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT4198600.46194014155308794No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTCC3959960.43568439073609444No Hit
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT3789270.4169046635028032No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG3759860.41366890406797346No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC3744620.41199216235471925No Hit
ACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGT3148670.3464242998866197No Hit
CTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGG2798090.30785263913644545No Hit
CTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCA2784390.30634533195327074No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2213970.24358634192213838No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCC1882680.20713701369484294No Hit
CGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTCCAA1823650.20064239011653617No Hit
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC1786840.19659246475794784No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1647720.18128614539128615TruSeq Adapter, Index 6 (100% over 50bp)
CACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAG1452410.15979766612516563No Hit
CAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGTATTG1299540.14297853845422281No Hit
CCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCA1292940.14225239046816784No Hit
CCTGGAAGTACTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGC1226400.13493149849966823No Hit
CCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGAT1180000.12982645811285756No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA1124550.12372571480577456No Hit
ACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTA1096510.12064068608926225No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG1088110.1197164977433741No Hit
GCCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGT1057100.11630470243313706No Hit
CCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGATA1000510.11007853356482637No Hit
CTCCTATTTCCAAAAATCCATTTAATATATTGTCCTCGGATAGAGGACGT988760.10878577010480428No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG953150.10486787165277134No Hit
TGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCAT948640.10437167052896712No Hit
ACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAG927410.10203589450715699No Hit
GTCAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGTAT927130.1020050882289607No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC926300.10191376961859319No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)