FastQCFastQC Report
Fri 24 Mar 2023
EGAF00003443085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003443085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118426251
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC43569343.67902721162726No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC41742263.524747228551548TruSeq Adapter, Index 12 (100% over 51bp)
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAG37420633.1598256031933323No Hit
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA27013862.281070267098128No Hit
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTG15324511.2940129296164244No Hit
CAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGGATTGG9865950.8330880963208064No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT9023610.7619602853087023No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCA5642850.47648641685026405No Hit
CTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGG5566920.47007483163509073No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCCA5143620.43433106735769245No Hit
GCCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC4593150.38784897446428496No Hit
GTCAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGGATT4408760.37227894683586665No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG4180250.35298339385918753No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC4100710.34626697758084063No Hit
CCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGATA3669170.30982742162461935No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTCCA3641770.3075137454110575No Hit
CCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGATAA3531080.2981670001526942No Hit
TCAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGGATTG3505750.29602811626621534No Hit
ACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGC3399040.2870174451439825No Hit
CTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCAT3391990.28642213794304777No Hit
CACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGG3313200.27976905221799175No Hit
CAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGTATTGG3276500.27667007714362246No Hit
GCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGA3215350.271506526031969No Hit
TACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGT2836710.23953388510120108No Hit
ATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCG2598180.2193922359325552No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC2459880.20771408190570856No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG2459710.20769972698029596No Hit
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG2283710.1928381571413588No Hit
CGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCA2086670.1761999541807669No Hit
CCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAG1983980.1675287348241734No Hit
TGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATC1907030.16103102005652445No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC1865660.15753770673699702No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCT1836520.15507710363980026No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC1761490.14874151508857608No Hit
CAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCA1702600.14376880004417264No Hit
CAAAAATTGCCAGTGCCGACTATATTGCAAGTCGTCACGGCGGGGGATTGG1645470.13894470069815854No Hit
AAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCAC1593100.13452253926369756No Hit
TCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGAT1529870.12918335141758394No Hit
TCCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC1506230.12718717237785396No Hit
CCGCAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA1482350.1251707275610709No Hit
GGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCC1424430.12027992003225704No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1300400.1098067353326924No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC1238130.10454861059479119No Hit
CACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGATAAG1232470.10407067601928899No Hit
GTCAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGTATT1231390.10397948002255006No Hit
AAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGGATTGGG1204490.10170802417784888No Hit
TTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGA1188580.10036457204070405No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)