FastQCFastQC Report
Tue 5 Sep 2023
EGAF00003443087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003443087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85333373
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC19432802.277280191420536No Hit
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA13744911.6107308918868122No Hit
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAG11670671.367656004878654No Hit
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTG4833570.566433721071825No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT4597830.5388079526634907No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG3087290.3617916286984226No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCA2848530.3338119542045994No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC2704920.3169826651525892No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC2228040.2610983161300796TruSeq Adapter, Index 12 (100% over 51bp)
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCCA2203720.2582483174548837No Hit
GCCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC1970900.23096473638748583No Hit
CTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCAT1693990.19851436084684007No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC1497980.17554444964925975No Hit
CAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGGATTGG1328660.1557022713727723No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC1243910.14577063536443122No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG1191290.1396042319808453No Hit
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG1137830.1333393911430174No Hit
TGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATC1114240.13057493930305555No Hit
CGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCA1034090.12118236554413475No Hit
CCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGATA972220.11393197828943197No Hit
CACCGTTCCTGGAGGTACTGCAATACCAGGTCGATGCGTGGAGTGGACGGA956260.11206166665883464No Hit
CACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGG903310.10585659141822508No Hit
CCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAG878360.10293276465234769No Hit
ACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGC873560.10237026491382217No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC858800.10064057821785621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)