FastQCFastQC Report
Mon 13 May 2024
EGAF00003443091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003443091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110020827
Total Bases5.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA33392993.035151699050581No Hit
ACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGT22963672.0872111786616547No Hit
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC22226092.0201711445052126No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC21148031.9221842424434785TruSeq Adapter, Index 5 (100% over 50bp)
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT11107881.0096161156832606No Hit
CAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGGATTG9477660.8614423521830098No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA7462770.6783052085220193No Hit
CTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGG5980410.543570718660386No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC5579800.5071585219042208No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCC5062400.4601310622760544No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTCC4531180.41184747684181655No Hit
GTCAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGGAT3893380.35387663464845615No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT3857190.35058725744717406No Hit
CACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAG3752480.3410699685069628No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG3680490.33452666193828917No Hit
CTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCA3465500.3149858162764037No Hit
GCCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGT3321670.3019128369213222No Hit
CAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGTATTG3101070.28186208780270305No Hit
ACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAG3032920.275667806060029No Hit
TCAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGGATT2949260.2680637912310912No Hit
TACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGG2689750.24447643899277363No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGA2526100.22960198254099654No Hit
CCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGATA2331970.21195714153284814No Hit
ATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCC2219200.20170726402556494No Hit
CCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCA2160750.19639463353606673No Hit
GCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGG2112520.1920109180782653No Hit
CCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGAT2073020.18842068874832218No Hit
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC1977690.17975596565912016No Hit
TGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCAT1887960.17160023710783415No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA1865450.16955426084917538No Hit
CAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGC1703280.15481432438241896No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1664770.15131407801542884No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1570850.14277751247952353No Hit
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAA1554730.14131233534537965No Hit
CGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGC1537520.1397480860601057No Hit
AAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCA1537510.13974717714128798No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1536980.1396990044439495No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC1509320.13718493499417161No Hit
CAAAAATTGCCAGTGCCGACTATATTGCAAGTCGTCACGGCGGGGGATTG1361210.12372293838511139No Hit
GGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTC1246820.1133258160293596No Hit
TCCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGT1234770.11223056885402251No Hit
CCGCAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC1185900.10778868259188781No Hit
AAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGGATTGG1143570.10394122923653355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)