FastQCFastQC Report
Sun 5 May 2024
EGAF00003443097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003443097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83690992
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC4291950.5128329701241922No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT3856780.46083573725592836No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA3143270.3755804447866982No Hit
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA2607930.3116141818464764No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2556590.30547971040897687No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTCC2169280.25920113361782116No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG2030000.24255896022836007No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC1695100.20254270614930694No Hit
ACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGT1529830.18279506114588773No Hit
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC1462210.1747153385396603No Hit
CTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCA1288150.15391740128973497No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA1172260.14007003286566372No Hit
CTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGG1145700.13689645356336558No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1110020.1326331512476277TruSeq Adapter, Index 3 (100% over 50bp)
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1102700.1317585051447353No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCC1090900.13034855650892513No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC982390.11738300341809785No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT953790.11396567028384608No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC863800.10321301962820562No Hit
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG861110.1028915991341099No Hit
CCTGGAAGTACTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGC844090.10085792745771252No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)