FastQCFastQC Report
Fri 24 Mar 2023
EGAF00003443099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003443099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111214886
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAG18929841.7020958866963187No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT12342001.109743528397808No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC7289480.6554410351146698TruSeq Adapter, Index 5 (100% over 51bp)
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA5897350.5302662451139859No Hit
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTG4014440.36096247043763546No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCCA3673710.33032538467916966No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG3528680.3172848641862565No Hit
ACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC2900580.2608086115378475No Hit
CAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGGATTGG2745800.24689140984238386No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTCCA2727890.24528101391031415No Hit
CTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCAT2533150.22777076802470492No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA2507990.22550848094202067No Hit
CAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGTATTGG2471700.2222454285481172No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCA2460870.22127163804313033No Hit
TGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATC2313370.20800902497890433No Hit
CACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGG2279410.20495547691340527No Hit
AAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCAC1963420.17654291350889845No Hit
CTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGG1921490.17277273475782728No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGG1853940.16669890755451566No Hit
ACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGC1800090.16185692983581354No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC1733890.15590448926054737No Hit
CAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCA1580420.14210507755229818No Hit
GCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCT1564520.140675412821985No Hit
CAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCA1420340.1277113209467301No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC1399300.12581948786963643No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC1196040.10754315748702922No Hit
CCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAG1155690.10391504604878163No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC1124150.10107909475355664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)