FastQCFastQC Report
Tue 4 Apr 2023
EGAF00003443100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003443100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101270062
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAG25813942.5490198672930604No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT13017691.2854430759605933No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC9903060.9778862384818131TruSeq Adapter, Index 7 (100% over 51bp)
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA8303430.81992938841096No Hit
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTG5509770.5440670116307423No Hit
ACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC5088370.5024555035820952No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCCA4374230.4319371306398529No Hit
CAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGGATTGG3865170.3816695599534638No Hit
CAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGTATTGG3520780.3476624710667206No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG3404210.3361516654349436No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTCCA3234720.3194152285598482No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCA3139800.3100422709329437No Hit
CACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGG2953670.2916627028430179No Hit
CTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCAT2858070.28222259802704575No Hit
TGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATC2759290.27246848135631635No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA2609570.2576842502574947No Hit
AAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCAC2545520.25135957752252586No Hit
CTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGG2525520.24938466019700867No Hit
ACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGC2377780.23479594591341318No Hit
CAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCA1890640.186692884615791No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC1838210.18151563884694766No Hit
GCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCT1785400.17630086964891956No Hit
CAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCA1756110.17340860322569962No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGG1621290.16009568553438824No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC1514040.1495051913763023No Hit
CCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAG1487230.14685781470144652No Hit
CGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCA1415940.1398182218946405No Hit
GTCAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGGATT1304820.12884558123406697No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC1180780.11659714398120936No Hit
GGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCCAAAAATCCATTTAATA1151330.11368907821938531No Hit
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATC1138490.11242118129640326No Hit
ACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTG1120970.11069115371925022No Hit
ATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCG1113200.1099238983382868No Hit
AAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGGATTGGG1087900.10742562792150756No Hit
CCTGGAGGTACTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCT1082510.10689338770228066No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC1080460.10669095867641516No Hit
CGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAAT1047340.10342049558535868No Hit
GTCAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGTATT1026700.10138238090542495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)