FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003443302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003443302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587435
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC235364.006570939763549RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT32220.5484862154961826No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG11950.20342676210985045No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)