FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003443443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003443443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472184
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTC9250.1958982091726954No Hit
CCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATAC8180.173237551462989No Hit
TTGTAACGCATGCTAACGTCAGCTGCCTTTGCAAGCAGTAGCATGTGAGGA6410.13575216441048404No Hit
CTTCAGACGCTACGCCGTTAGATCAGGTAATCGATCAACGCCGGTTCTTGT6390.13532860071497552No Hit
CTTTTGTTTGAAAGCTGTCCCTGAGAATTTGATTAGACGCTTCCCCTAGAA6350.13448147332395846No Hit
GTGTGATTGGTGGAGTCTTGGAGCCCGCCCCTCGTCGGGCCCAACAATGCG6320.13384612778069566No Hit
GATACATCATTGTCACTGGGCCACAAAAGACACATGTGGCTCTGGGGCTAA6300.13342256408518713No Hit
TGTTTTGATGTTGAAAGGAGAGCCTCTACAACATTCCAAGTACATTTAATT6290.13321078223743288No Hit
GGGCACATGCAGAGCAGCCCAGAGGCTAGGGGGCTCACAAGCTGGGTTCCT6220.131728309303153No Hit
GTTTTAGGCGGCCTTCTGCTCCGCCCCCGAAGCTCCTGCAGGGCTGGCACC6180.13088118191213596No Hit
ACATGAAATAAGGCAGCCGGGTGGAGTAGTAGGCACCTGTAGTCCCAACTA6170.13066940006438169No Hit
GCCCAGGACCGCTGTTAGATTGGAATGCCTTCATTTTGGCCTGCCTCCCCG6160.13045761821662744No Hit
AGGACGCTCTGCGCGCGGTGCGCCATGGGACGGGTAGTTCGCGGTGCGCGC6140.13003405452111888No Hit
TATTGGCTGACTCTCGGAAGACCCGCCTCTTGAGAAGGAAAAGCAGTCGCT6120.12961049082561035No Hit
GTATAGCACACAGAGGTGTTCATTTGTCTAATGCAATTGGCCAGATAGAGA6020.12749267234806771No Hit
GGCTCTAACTCACATAGCTTCACCTTGAGGGACAGGGCCAGAGGCTGGTCC6010.12728089050031344No Hit
TTTTACCTCACCCCCACTGATGTTCGCCGACCGTTGACTATTCTCTACAAA6000.1270691086525592No Hit
TGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGTCATTAAGG5980.12664554495705063No Hit
GTGAGTGCATGTGAGGAGCTGGACCCAGGGTCCTGGGGGACACAGAGAGGA5960.1262219812615421No Hit
TACAAAACCCACCCCATTCCTCCCCACACTCATCGCCCTTACCACGCTACT5950.12601019941378783No Hit
TCCATGAAGGGAGCCAGCCCTAGGGCAAAGCCAACTCACAGAGAATGTCAG5950.12601019941378783No Hit
GAACAAGCATTTAGCCCAATAGAAAGTCGTAGTTCTCTTGCTGGGCGTGGC5930.1255866357182793No Hit
TTTGGTGATTCCATTCTTTTCCATTTGATGATGATTCCATTAGGGTCCATT5930.1255866357182793No Hit
CAGGAACGGTATGGAATGGAATGGAATGGAATGTTACGGAATAGAATGGAA5910.1251630720227708No Hit
GGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCT5910.1251630720227708No Hit
CTTATTTACCGAGAAAGCTCACAAGAACTGCTAACTCATGCCCCCATGTCT5890.12473950832726226No Hit
ACCTAGATCTCCATCTGTGTCTGCCTATTTACTGCTTATGATTTCAAGTTG5890.12473950832726226No Hit
GATTGAAGAGTATGCAATGAGCGATTTTAGGTCTGTTTGTCGTAGGCAGAT5870.12431594463175372No Hit
CGATGTGAAACCTGTGGAACCATAGAATGAACACGAAATGAATCATAATCG5860.12410416278399947No Hit
TGTCAGTCTCCTCCAGTAAACTATATTCTTTGAAGTCATCTAGTGTCTTAT5860.12410416278399947No Hit
GTAACAGAGCTCAACAGATACTGTGCTTTCTGTGAAACATTTCCCTCCAAA5850.12389238093624519No Hit
GACAAGTAGCATCCGTACTATACTTCACAACAATCCTAATCCTAATACCAA5830.12346881724073666No Hit
CACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAA5830.12346881724073666No Hit
CAATCGAGTTGATTCCATTCCATTCCATTCCATTCCATTCCACTCCATTCC5830.12346881724073666No Hit
CTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCC5810.12304525354522813No Hit
TTATCAATCTACAAAGTTTATGAATATGCATGTAGAAACCATGACACAAAG5810.12304525354522813No Hit
AATCCCAGCACCTTGGGAGGCTGAGGCAGGAGGATCACGAGGTCAGGAGAT5770.12219812615421106No Hit
GGGCTAGGATTTTAACATATAAATTTGGGGGGAGACGTAAATTCAGTTCAT5750.12177456245870255No Hit
TCCTAATTTAGTCAGGGGTACAGCATCCGAAAAAGAAGCCCAAAAGCTCGG5740.12156278061094827No Hit
TTCAGGGGCTGCACACGTGCAGGTTTGTGACATGGGTCTATTGTGTAAATA5740.12156278061094827No Hit
GGGCAGACCTACAAAGTTTGCCAGAAAAATCAATACAAAGCTGACAGATCA5710.12092743506768547No Hit
CATTTACATTGTTATTCAGCCATCATCACCATCCATCTCCAGAACCCTTTT5700.12071565321993122No Hit
GAGTGCACGGATGCAGAACCCATGGACATGGAGGGCTGAGTGTATGTCCAC5690.12050387137217694No Hit
TTCAAGTCCCACCTATGAGTGAGAACATGCAGTGTTTGGTATTTTGTCCTT5680.12029208952442268No Hit
GGCTAATGGGCCAGAGGGTTCAACAGTGGAAGAGGGACAGTGTGGCCCAGC5650.1196567439811599No Hit
GACTAGAAGAGGCGTTTTTATGTTCACTTGCTGATACGCACGCTGTGGTAA5650.1196567439811599No Hit
ATACGGATGTGTTTAGGAGTGGGACTTCTAGGGGATTTAGCGGGGTGATGC5640.11944496213340562No Hit
GCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATA5640.11944496213340562No Hit
GCCACGCCCGGCCAACATCTGATTCTTTTGATGCAACCTCATGGTTGTCGT5620.1190213984378971No Hit
ACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAGCTAAGCCCTT5620.1190213984378971No Hit
ATTCTTCTCTGATGGTCACCACTACTGGAAGCAGACGAGAGAGGTTAACAC5600.11859783474238855No Hit
GAATAGGAGGTGGAGTGCTGCTAGGGCTGCAATAATGAAGGGCAAGATGAA5600.11859783474238855No Hit
GCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGGACGATGGGCATGA5580.11817427104688003No Hit
TGAATGAGTAGGCTGATGGTTTCGATAATAACTAGTATGGGGATAAGGGGT5570.11796248919912575No Hit
TTTATTGAGAGGCTACTGTGTTGCAGGCACGGTGCCAGGTTTGACACAGTG5570.11796248919912575No Hit
GTGGGGAGGTAGTAAGAAGGGGAGCCACTGCATGCCTGCCCATTTCACAAA5550.11753892550361723No Hit
GGTCGTGGTGCCCTGTCACGGCCTGCTCAGCCACCAGGAGCTGCTCATGGA5540.11732714365586298No Hit
ACTTATGACTCCCTAAAGCCCATGTCGAAGCCCCCATCGCTGGGTCAATAG5530.1171153618081087No Hit
CTTCCATATACTACAAAAAGAGCGTTTCAAACCTGCTCTAGGAAAGGCAAT5530.1171153618081087No Hit
GTTCTAGAGGCTGCGAAGTCCAAGATCAAAGCCCCTGGCAGATCTGGTGTC5520.11690357996035443No Hit
CTGATGGGATAATCCCTTTGGATCCTCTCTCATACAGTATAAAAGAAAAAT5510.11669179811260018No Hit
GGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTTTTCTCGTGTGA5500.1164800162648459No Hit
TCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGA5500.1164800162648459No Hit
ATATCACAGGGGCCAAAAATAAGTGTCTACTGCTAACAAGTGGGTGCGTGA5500.1164800162648459No Hit
GTGTAGCATGTCATAGAACTGAAGGAAGGATTGTGTATGGCGAATAAAGGG5500.1164800162648459No Hit
AGACAGCCCCATGTATGGGCTAATTCAGTTGGTGTTTTCACTCCTTTCCCT5490.11626823441709164No Hit
GCGTGATATTGGCTCACTGCAACCTCTGCCTCCCGGGTTCAAGCGATTCTC5450.11542110702607458No Hit
CGGTCAACTGGTGCCCGAACGACCAGACCGTACTGGCGAACGAACAAGTTA5430.11499754333056605No Hit
GGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCCTGGAGCC5420.11478576148281179No Hit
GGGCCTTGGTGATGATGCGCGCACCCTTCTCGTTCAGCTGCTTGTAGTCCT5410.11457397963505751No Hit
GGCTGGAGTGCAATGGTGCCATCTCGGCTCACTGCAAGCTCCACCTCCCAG5410.11457397963505751No Hit
CCCCATACCCAACCCCCTGGTCAACCTCAACCTAGGCCTCCTATTTATTCT5400.11436219778730326No Hit
AAAGGGAGGAGACACCACCGAATCCTGGGACTTCTGAATTGGTTGTCTCAT5390.11415041593954899No Hit
GGACCCGGACGGCCCCGAGATCGTCGTGGTCAACCCCGAGGGCGCGCAGAC5380.11393863409179474No Hit
ACCATCTGCCCTGTGTGCTTTAATTATCCCATTTAATACTCACGGTGACCT5380.11393863409179474No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCC5380.11393863409179474No Hit
GACCGTAGGAGATCGTCACGGCGTCGAAGGAGTTGTCGGCGTAGGGCAGGC5370.11372685224404046No Hit
GCTGAGAATCCTCATTTCACCCCCAGAGAAATGTTCTTCTTTGACCTACTC5360.1135150703962862No Hit
CTTTCATGCAGCAGGTTTTAGAATAAAAGCCATGTGAGATCCTATTGAATC5340.11309150670077767No Hit
GAGAAATGATCCGTAATATAGGCCTCGCCCGATGTGTAGGAAGAGGCAGAT5330.11287972485302339No Hit
GTATTTCAATGGTCCCGTGGCTCTGGGCCACGCCCACATGACTAGCAGCCA5330.11287972485302339No Hit
GTATGGTGTCCCATAAGTCTTACAAGCTTTCTTCACTCTTTTTCATTCTTC5320.11266794300526914No Hit
GGGTAAGAGAAAGCTAGCGCAATCACTGTCCTTCTCCAGCAAAAACACAGT5320.11266794300526914No Hit
CCTCAGCTGAGCGGCTGCGACCGTAATCCTTGGCTCTTGAACTTTGGGTCT5310.11245616115751486No Hit
CCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTAT5300.1122443793097606No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACACTGTC5300.1122443793097606No Hit
GGCCGTTCTCTACTCCGACATCATGGTGCCGCTAGCCCTGGCGGGGGCAGA5290.11203259746200633No Hit
ACCTGGACGGGCTCTGAAATACAGCCAGCGGAATGGTACCCTCCCCAAATA5280.11182081561425207No Hit
CTAGAAGGTTGCCTGGCTGGCCCAGCTCGGCTCGAATAAGGAGGCTTAGAG5270.11160903376649782No Hit
GTGTATATCCAATGTGTATTTTCATAATTTCCCTTTCTCTCTTTTCCTGCT5260.11139725191874354No Hit
ATAGATAGTTGGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGAGG5220.11055012452772647No Hit
ATGCTGCTCTCAAGGGCATACATGGTGATTCTCCTGCCCAGGCATTGGAGG5210.11033834267997222No Hit
AGACTGTAGTGCAGTGGCGCGATCTCAGCTCACTGTAACCTCCACCTGTCT5200.11012656083221795No Hit
GTTAAGAGGTGGAACATTTAAGATGTGATTAAGTCATGAGGGATCTGACCT5190.1099147789844637No Hit
ACCAGTAAGCTACCCTTTTACCATCATTGGACAAGTAGCATCCGTACTATA5190.1099147789844637No Hit
ATCTTGGCTCACTGCAATCTCCACCACCCAGGTTCAAGCGATTCTCCTGCC5180.10970299713670942No Hit
ACCCTGACTCGATGGCCGCTTCGTTGACAGCGATGGGGCATGTCTTGGCGA5180.10970299713670942No Hit
GCATTACATTCGTGTTCATTCCATTCCAGACCATTCCATTTGACTCCATTC5180.10970299713670942No Hit
AGTTATTATCTCGAACTGACACTGAGCCACAACCCAAACAACCCAGCTCTC5170.10949121528895514No Hit
GTGTTTTAGTGGGGTTAGCGATGGAGGTAGGATTGGTGCTGTGGGTGAAAG5160.10927943344120089No Hit
GAGGAGGGAGAGATGCAAACCCAGCAATTTCCCCACAACCACAACACCTGT5150.10906765159344663No Hit
TTACTGGAGTAGCCATTGACCCTCACCAGTCCTGTCTCATTGACTTATGAA5140.10885586974569235No Hit
AGACTAGAGGACTTAAACTGTCCTTGGAATCCACATTTGTGGCCTTGGTGA5130.1086440878979381No Hit
TACCTGGCCATTGTCCATGCTGTGTTTGCCCTTCGAGCCCGGACTGTCACT5120.10843230605018382No Hit
CACTATTTAAGCAGCCACTCCTACCTCCTGCACCTACCAGTACTCTCAGAT5100.1080087423546753No Hit
GACCATGGCTGTCCTGTTCCTACCTGATCCACAAAGCCCAGAACAGAGCCT5100.1080087423546753No Hit
CAGCAGCCCTGACGTTGTTTGCCCGGGACGGTTTTGACAGCGTGACCGTGG5100.1080087423546753No Hit
GATTCCAGAGAGACGGGCTGTGGCCTTTAGCGCCGCCACTCGAAACCTGTC5090.10779696050692103No Hit
GCCATGCGTGCGCCCCCACAGCTCTAGGACCAATAGGCAGGCCCTAGTGCT5090.10779696050692103No Hit
GCTCAAACTGGCCCCAAAGTGCTCCACAGAATGCTGGGTCCAAGGTCAGGA5060.10716161496365822No Hit
GTAGAAGAGCGATGGTGAGAGCTAAGGTCGGGGCGGTGATGTAGAGGGTGA5050.10694983311590398No Hit
TAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCC5040.1067380512681497No Hit
CCCCGGACCCTGTCCACCCCTCTAGGGTTCTCGCGCGGGAAGAGCCGTTCC5030.10652626942039543No Hit
CTCTAAAGCAAGGTTCAACTCTGTGAGTTGAATACACACAACACAAAAAAG5030.10652626942039543No Hit
ATATATTCTTCAGCCCCAGCTGACCCAGGCTAAATGTAAGTGATCACTCAG5020.10631448757264117No Hit
TGGTGGAATTACTAGATGAACCCTCACAAAAGGTGTGAAGCTGTCTCTTAT5020.10631448757264117No Hit
TTCCAAAAACACAGTGTTTCAAATCTGCTCTCTCTAAAGGCTGTCTCTTAT5010.1061027057248869No Hit
CCCCTCCCCATACCCAACCCCCTGGTCAACCTCAACCTAGGCCTCCTATTT5010.1061027057248869No Hit
AACGCACACATCACAAAGGAGTTTCTGAGAATCATTCTGCCTAGTCTTTAT4970.10525557833386985No Hit
TACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGC4960.10504379648611559No Hit
ATAGCAAAGACCTGGAACCAACCCAAATGTCCAACAATGATAGACTGGATT4960.10504379648611559No Hit
GTGCAATAGCTCATGCTGGTAATCCCAACACTTTCAGAGGATTGCTTGAGC4960.10504379648611559No Hit
CTATTACCTAAAACAATTTCACAGCACCAAATCTCCACCTCCATCATCACC4960.10504379648611559No Hit
TCCCCACTGATCCCAGCAAAGCAGCTGTGTTGTTCATGCGCCGATTGAACC4930.10440845094285278No Hit
GAGTTTGATGCTCACCCTGATCAGAGGATTGAGTAAACGGCTAGGCTAGAG4930.10440845094285278No Hit
GGGTGAGGTGTGTAGGAGGGTTTGGTGTGTAGGAGGGTGAGGTGTGTAGGA4930.10440845094285278No Hit
AGCTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTAAGTGTAAGTTGGGTGC4920.10419666909509853No Hit
GCCACCACAGCCCGCAGGGGCACCACCTGTGGGAGACCAGGCAACCTTGTC4910.10398488724734425No Hit
TCTTTGTCCTCATCAGCGCCACTCATTGTCTCTCAGTATGACCTGGACTTG4910.10398488724734425No Hit
GCGCAGGCGCGAGAAAACCCCGAGAGAGACCAGTGTCTCTGCGCATGCGTC4900.10377310539958998No Hit
CCCCAGCCCTGGCCCGGGCCAACGCGCCGCCGCCACCGCCTATCAGCGGAG4890.10356132355183573No Hit
AGTTTAACCAAAACATCAGATTGTGAATCTGACAACAGAGGCTTACGACCC4880.10334954170408146No Hit
CAGCTATACATGGGCCTTGAAAAATGTTAAGGACACACACTTTGGGGACGC4880.10334954170408146No Hit
AGGCTGTGCTTGAACGCAGCGATCGCCTCTATGGCCTGGCCCACACGTACC4870.10313775985632719No Hit
GGGGTACATGTGCAGAATGTGCAGGTTTGTAACATAGGTATACACGTGCCA4870.10313775985632719No Hit
AGAAAAGGCAAATTCTTTTTTTCTCTCTCCTGAAGCTCTGTCTCTTATACA4860.10292597800857294No Hit
GCATCGGACAGGTGTTCTTCACCCAGAGACGGGACGGGGAGAAGGCCTGTC4850.10271419616081866No Hit
ATATACTACAGCGATGGCTATTGAGGAGTATCCTGAGGCATGGGGGTCAGG4850.10271419616081866No Hit
TCCCAACACACAATCCTCCAACAGCAAGCAAAGCGCAGCTACTCCAACCAC4850.10271419616081866No Hit
CTACAAAGGGATCATGGGATTAAGGTGAGACACTGTCACCATGAGGTGGGT4840.10250241431306441No Hit
CAATACGCGAAAACCCGCCATAACATCGGGCAGATGGTGCTCGATAAAATC4830.10229063246531013No Hit
AGGTATTGATGACAGGAGAGTATATGGGTTTGGCACTATGGGGTGGCTCTG4830.10229063246531013No Hit
GTTCCAACTATGGCCAGGTAGTCAGTCCCATCCCTGCATTCCTAAGGGATG4820.10207885061755587No Hit
GGGTCATCTCAAGGGCTCTGAGCATCTGTAAGCAGGGTCCCCACATTCCTC4820.10207885061755587No Hit
AGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACTT4820.10207885061755587No Hit
GTATGTGGTATTTCGTGACTGGCTTCTTTCACTTAGCATAGTGTTTTAAAG4800.10165528692204734No Hit
GAGTACACTCACATTTCTTAATTGTGATGGGCAAAGGGCAGGGCTGAGATA4800.10165528692204734No Hit
GCCTCCGCAGGGTTACCCGGGCGCGTGCAGGGAAACGTCTCAGCAATTCCA4790.10144350507429306No Hit
ACCCACCCTGGCCGTCCTATGGTGCTGACCGACGTTAATGTTTCCTTTCGC4750.10059637768327602No Hit
CCCCGGGAGGGAGGACGGGGCGCGGGCGCAGCCTAGGGGCCGAAGCACTGC4750.10059637768327602No Hit
CAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCACTCCTAAACACAT4740.10038459583552174No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)