FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003443666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003443666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320151
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC133734.177091434979119RNA PCR Primer, Index 27 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT13540.4229254320617459No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG8150.25456737601944085No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)