FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003444705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003444705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440198
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC122772.788972235221423RNA PCR Primer, Index 27 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT33510.7612483473346086No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG11310.2569298361192009No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)