FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003444751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003444751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253509
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC212138.367750257387312RNA PCR Primer, Index 27 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT30931.220075026922121No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG9580.37789585379611773No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGC2550.10058814479959291No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)