FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003447666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003447666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7606690
Sequences flagged as poor quality0
Sequence length65
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC2540363.339639186032295No Hit
CGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGA942531.2390803358622475No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGA644250.8469518279304139No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTG558640.7344061608925827No Hit
CATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAA469390.6170752324598479No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCT379350.49870574454854877No Hit
GGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTT335760.4414009247123256No Hit
AGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACG333670.43865334330701006No Hit
CAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAACG283450.3726325116443552No Hit
ATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGA235550.3096616268048258No Hit
ATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAA234760.3086230673262615No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTT231960.3049420970224894No Hit
GAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCA160460.21094589105116682No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAACTTC158020.20773818835787972No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTC127650.16781280688446618No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTGGGCTCTTCCCTG116780.15352275431232246No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTC112180.14747544595612547No Hit
AGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTC104220.13701097323540198No Hit
CAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGG100800.13251493093579467No Hit
GCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAACGCTACC97140.12770337689586403No Hit
GAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGC94720.12452196684760389No Hit
TCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAA93540.12297070079101421No Hit
CGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCA87830.11546415063582188No Hit
GAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTC87750.11535898005571411No Hit
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAG78690.10344841185850875No Hit
GATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAG78410.10308031482813154No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)