FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003447693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003447693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12335642
Sequences flagged as poor quality0
Sequence length65
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC5444684.413779193656884No Hit
AGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACG879640.7130881392310185No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGA863320.6998581833033093No Hit
CGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGGA837600.6790080321721399No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTG691320.5604248242612747No Hit
GGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTT584670.4739680350645714No Hit
CATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGGAGAAA408690.3313082529470294No Hit
GAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCA311680.2526662171291936No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAACTTC295530.2395740732423979No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTC284950.23099730034318441No Hit
GAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTC275920.22367704899347757No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCT259320.2102201085277929No Hit
AGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTC250620.20316737466927137No Hit
ATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGGAGA246940.2001841493130232No Hit
ATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGGAGAAAA241290.19560392560030518No Hit
GAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGC233590.18936185080598156No Hit
CAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGGAGAAAACG230710.1870271527010917No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCC220180.17849091275508805No Hit
CAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGG219950.17830446117032256No Hit
CTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCG212390.17217587864498662No Hit
GAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCAC212090.17193268092572725No Hit
CCTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGC183900.14908020190598917No Hit
GCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCA175320.1421247471351714No Hit
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG159590.12937308005533882No Hit
AAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCT148890.12069902806842157No Hit
AGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCA133410.10815002575463846No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)