FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003447706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003447706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6082112
Sequences flagged as poor quality0
Sequence length65
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC995651.6370135900160996No Hit
CGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGGA952671.5663473477634087No Hit
CTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCG822131.3517179558679617No Hit
CCTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGC668521.099157661023013No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAACTTC613341.0084325970978503No Hit
CATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGGAGAAA521400.857268001641535No Hit
TGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGC371460.6107417949554366No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTC345360.5678290699020341No Hit
CCAGAAAGCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCT336020.5524725621626172No Hit
GCCAGAAAGCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTC325780.5356363052834279No Hit
CAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGGAGAAAACG302610.4975409857628402No Hit
CAGAAAGCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTA300090.4933976881714773No Hit
GCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCA299490.49241118874496226No Hit
AGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACG299080.4917370808035104No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTG298230.49033953994928076No Hit
ATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGGAGAAAA272250.4476241147811813No Hit
CAACGCCAGAAAGCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGT269080.4424121094777604No Hit
ATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGGAGA254910.41911428135489776No Hit
CGCCAGAAAGCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCT236750.38925623204571047No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGA205130.33726771226836993No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTGGGCTCTTCCCTG188550.31000744478233877No Hit
GGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTT186760.30706438815990234No Hit
CAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAACTTCTC183880.30232919091263033No Hit
GAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCA174330.28662740837393325No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCC173900.2859204171182642No Hit
CACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAA165270.27173126703355677No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTT160980.2646777961339745No Hit
GTTTCCTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGC150320.2471509896562247No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTT145060.23850267801710984No Hit
CATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAG114480.188224090579062No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCT108290.17804670482884893No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTC107960.1775041301442657No Hit
AGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTC102720.16888870181936802No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTT97330.1600266486378416No Hit
CGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAACT92400.15192091168331No Hit
CTCCTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAG92310.15177293676933276No Hit
CCCGTCCCTGCTGACACCAACCATTCTTACCGGTAGCCTGCCAACACACAAGCAGTTCACAGTAG87850.14443995769890458No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTGCTGAGCCTGGGGCAT87530.14391382467142994No Hit
GATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGGAG87200.14337124998684667No Hit
CTTCCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGACTCTTTTCCTATCTTCACC85330.14029666010754158No Hit
CCCTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGG85100.13991850199404418No Hit
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAG81510.13401594709206277No Hit
CCCCGTCCCTGCTGACACCAACCATTCTTACCGGTAGCCTGCCAACACACAAGCAGTTCACAGTA81160.13344048909326234No Hit
GCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAACTT79510.1307276156703461No Hit
GAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTC76680.12607462670861702No Hit
GAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGC71620.11775514821167385No Hit
CCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTG67990.11178682668125808No Hit
CTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTT63350.10415789778287542No Hit
GAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCAC63220.10394415624046384No Hit
CTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTC62790.10323716498479477No Hit
GCCCCGTCCCTGCTGACACCAACCATTCTTACCGGTAGCCTGCCAACACACAAGCAGTTCACAGT60920.10016257510548968No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)