FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003447793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003447793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6093
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN195432.06958805186279No Hit
ACCCCAGCAAAAGCAACCAGGAAGTTCTGGAGTTTGTCACCAGTGGAGGC390.6400787789266371No Hit
CCGGAAAGTCCACGCTCACCATGTGTGACTTGATTAGCAGGTCAAAAGTG360.5908419497784343No Hit
GTTCCCTACAGATTGCGAGAGAGCTGCATCAGTTCACTTTTGACCTGCTA340.5580173970129657No Hit
TTCATGGTCCATTCGTGTAGTTGAACCCTAAAAATTAAGCACATTATAGA330.5416051206302315No Hit
CCCCATAAGCATGACGACCTATGATGATAGGTTTTACCCATCCACTCACA290.47595601509929425No Hit
ACCTTAACATGATGTTATCTGTATTTACCTATAGTCTAAATTATACCATC240.3938946331856229No Hit
GCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCC220.36107008042015426No Hit
AGCAGCCAGAAATGTTTTGGTAACAGAAAACAATGTGATGAAAATAGCAG220.36107008042015426No Hit
CCTAAAAGAGTCATACACAGGCCCAGGGCAGTTCTTGGGTGGGTCCATCC210.34465780403741997No Hit
AACAAGGGGTGCCGCAGCCCCAGATCTAGCCCCAAGTGAGACCTGAGGGC210.34465780403741997No Hit
GTGCGTGGCTCTCACCACCCGCACGTCTGTAGGGGAGTACATCAAGACCT210.34465780403741997No Hit
CCACGGCCGGCCCTCTAGGGACTCGAACAGAGACCTCTGCAGCTGTGGGT190.3118332512719514No Hit
ACAGAAGCGGGCAAAGGGGCGACCGGACCCGAGCCCAGGGAGGCGTACAT170.27900869850648286No Hit
GAGCCTAGGAACCCCTTGTTACCCACCCCCCAGCTCCCCAGCCCTCAGGT170.27900869850648286No Hit
CGAGGTCATAGTTCCTGTTGGTGAAGCTAACGTTGAGGGGCATCGTCGCG170.27900869850648286No Hit
GGGTCCGTAAAAATGCTGGAGACATCTCACATTGTTTTTGTTGACGATCT150.2461841457410143No Hit
GAGGGGAGCCTCACGTTGGTCCACATCCTGCGGCCGCTCCTTGTAGCCAA150.2461841457410143No Hit
ACGAAATATTCTGGGTGGCACGGTCTTCAGAGAAGCCATTATCTGCAAAA150.2461841457410143No Hit
AAACTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGTGCCTTGACGAT150.2461841457410143No Hit
CCTCACTCCAGGTCAGTGAGCCCACCTGACTTGGTGCAGGCTCCCAGCAA140.22977186935828No Hit
AGAAACAGAACTGTATGTAAGCATTTTATCTTTGGCCTTTGC140.22977186935828No Hit
GCTTTTGCCTTCCATTCTCCCTGTAAGGGTCCACGTGAACAGCAAGTTTG140.22977186935828No Hit
AAAACATGGTAAGAAACCATTCTTTTCCCCCATTCTTTCTGTAAACAGTT140.22977186935828No Hit
ACAGTTGCAGGCACTCAGAAATAGGCTTGCCTTTCTTCAAATTCCATTTCCAAATCGAAGTGGATTA140.22977186935828No Hit
GCAGCGCAGTCTGAGGAATAAAATTGGAGAAAGTTGGTGGCTAAACCGGG140.22977186935828No Hit
TAATCCACTTCGATTTGGAAATGGAATTTGAAGAAAGGCAAGCCTATTTCTGAGTGCCTGCAACTGT140.22977186935828No Hit
ATTCATCTACAAAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTC130.2133595929755457No Hit
AGAATATGAAGAAGAACACAAACCAAGTAGCTGTGGGTTGAACCTGGACG130.2133595929755457No Hit
AAAGCGGCTGGGTGGTCTTGGTGGGGGAATAAAGGGAGCACCCTTTCACC130.2133595929755457No Hit
GCAAAGGCCAAAGATAAAATGCTTACATACAGTTCTGTTTCT130.2133595929755457No Hit
GCAGTTTTATCTCTGGCTTTGATGCTGTTATGTTGCTTTTGAAGGCTTCT130.2133595929755457No Hit
GCTTGGGAATGTGCTTTGCTTTGGGTGTGTCCAAAGCCTCATTAAGTCTT130.2133595929755457No Hit
ATGCATATTAAAACAAGATTTACCTCTATTGTTGGATCATATTCGTCCAC120.19694731659281145No Hit
CTCATGTCTGAACTGAAGATAATGACTCACCTGGGGCCACATTTGAACAT120.19694731659281145No Hit
GGGGCGCCAGGTGGCTGAGTCCTGGCGCTGTGTCCTTTCAGGATGGTGGA110.18053504021007713No Hit
CACTAAGTGCGTCTCCGAGATAGCAGGGGACTGTCCAAAGGGGGTGAAAG110.18053504021007713No Hit
GAGAGTCAGAGAGGCAAAAGAACCACAAGTGGTATCAATACTAGAAATTT110.18053504021007713No Hit
CTCAACAGGCAAAACGGGAGGAGGAGCGCAGCCCAGCTGCCCCACCACCT110.18053504021007713No Hit
GGTCCTGTGTGAAATTATGTTTGTTCCAACTTACTCAATTGAACTAATCA110.18053504021007713No Hit
CACAGAGACTTGGCAGCCAGAAATATCCTCCTTACTCATGGTCGGATCAC110.18053504021007713No Hit
TCAGAGAGAAGAAACCAATTGGTTGGGACACTGATATTTCCTGGCTTACT110.18053504021007713No Hit
TCTCCCTCTTATTGTTCCCTACAGATTGCGAGAGAGCTGCATCAGTTCAC110.18053504021007713No Hit
GGAGAGAGAACAAATAAATGGTTACCTATAAATATTTGTAGGTCAGAATC100.16412276382734287No Hit
CAGTCTCGCTTATTTAGACTGAAATTGCACTTACTCCTTTTGCAATAGAG100.16412276382734287No Hit
CAAAAAGGGATTCAATTGCCATCCATTTAACTGGAATCCGACCCTAAAGA100.16412276382734287No Hit
CCCCTCCCCAGAGGGCAGAGCCCCCCAAGCCTACTTCAGAGCTCCCTTCT100.16412276382734287No Hit
GAAATCATCGCAGAAGGATAAAGTTCGTCAGTTTATGATCTTCACACAATCTAGTGAAAAAACAGCAGT100.16412276382734287No Hit
GCTTGATCTGAAGGGGACCGGAAAGCTGAACAATTGTGGAATTTGTTCTA100.16412276382734287No Hit
GCAGTTACTGGACTTGTCCATGCGGTGACCCTCCTTCAGCAGCTTGAAAA100.16412276382734287No Hit
GGTGCTAGTGGGAAACAAGTGTGATTTGCCAACAAGGACAGTTGATACAA90.14771048744460857No Hit
CCTAGAGATTGAATAGATCTTGAACTTTTTTTTTCTCCAGGGGGGGAAAA90.14771048744460857No Hit
CAAAGCAGTGGATCCACTTCCTCCGCTCCGACCGCTGGCCCCCCACATCC90.14771048744460857No Hit
GACTCCCGGCCGGTCGGACATTCCTGCTTTATTGTGTTAATTGCTCTCTG90.14771048744460857No Hit
GCAGGAGGATAAAGACCTGGTCCATGGAAATGTGTGTACTAAAAACCTCC90.14771048744460857No Hit
CAACACAGTGGAGCGAATTCCTTTGGAAAACCTGCAGATCATCAGAGGAA90.14771048744460857No Hit
CCACGGCCGACCAGCTGGAGATGGTGACCGAGCTGCTGGGAGGAGACATG90.14771048744460857No Hit
GAACTGAAGATAATGACTCACCTGGGGCCACATTTGAACATTGTAAACTT90.14771048744460857No Hit
AACAGGGTGTTGATCTTAGAGTCCAACAAATTATGCCTAAAAAAAAAAAA90.14771048744460857No Hit
GCACAGCTTCTCGGCCGTCAGGGGGATGGTCTCCTTCATCTTAGAGGCCA90.14771048744460857No Hit
GAGCCTAGGAATCCCTTGTTACCCACTCCTCAGCTCCCCAGCCCTCAGGT90.14771048744460857No Hit
GCAGGTTGACAGCTTCCAACTGGCTGAAGGTGGGTTTGATTTCAATGAAA90.14771048744460857No Hit
ACATTGGTGGTCTTTTTGTAATAGTTTATATTGTTGATATCTCTGGCGAG90.14771048744460857No Hit
CAGGGATAGGGCCTGGCCTTCTCCTTTATCCCTCCTTCCTAGAGAGTTAG90.14771048744460857No Hit
AGGGCAGTCTCTGCTAAGGACGCCTCTTCCTGTGGTATTGGGTGGTGTTC90.14771048744460857No Hit
CACCCCTGCGCTAACAGGTTTCTGTCCAGGCAGTGCACCCAGCAACCCCC90.14771048744460857No Hit
GTCCTTACCACACCATGGAAATTCAAAGTTGCCAAACAGCTGGCCAGTGCCCTG90.14771048744460857No Hit
ACACCCAGTGGAGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGA90.14771048744460857No Hit
GAGTACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACT90.14771048744460857No Hit
CAGAAGGCTGCAGTCAACTCACATATATGCCCTTTGTTTCTGACATAGAA90.14771048744460857No Hit
TAGATAAGACTGCTAAGGCATAGGAATTTTCGTAGTACATATTTCCTCTG80.13129821106187428No Hit
GTAGTTTTCTATTTCCTGGATAACGTCCTGTAGTTTTCTATTTCCTGG80.13129821106187428No Hit
GCCGGAAGAGCCTCCACCATCTCCATATCATTGAGACCAGGAG80.13129821106187428No Hit
GCAGCAAATTCAACCACCAGAACATTGTTCGCTGCATTGGGGTGAGCCTG80.13129821106187428No Hit
GATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGT80.13129821106187428No Hit
GGGAAGGGACTGCACTTACCTCACGCCAGCCCGGGGTGCTGTGGGGGCGG80.13129821106187428No Hit
GGGCTGAGATGATGAAAAAAATATAAAACAGAGAAGCTGGTCGGCCGTGG80.13129821106187428No Hit
ACAGAAATCTCCAGGACCTCAGCGAGAAAGGAAGTCATCTTCATCCTCAG80.13129821106187428No Hit
AGACCCTCCTAACTTTTGGGGTTTTTTTCCTTTCCTCAGGGTCAGTTTGA80.13129821106187428No Hit
GAAATTCACATATATATCCATGTTAATATGTATGCTTATCTGTCTCTTCC80.13129821106187428No Hit
CACCAGAATTTATTTTTAATTGTCATACTAATTTGGTGATCAGACCCTCT80.13129821106187428No Hit
ACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATGGAATCCCGTAACTCTT80.13129821106187428No Hit
CCAGGAAATAGAAAACTACAGGACGTTATCCAGGAAATAGAAAACTAC80.13129821106187428No Hit
AGCCTGAGTTGGGTTTGGGTTGGTTAAACAGGATCCAGTAGATAATAGCC80.13129821106187428No Hit
CGATCTGCCATGTGTGCATTCCCTATCAAATATGTCAACGACTTCTTCAA80.13129821106187428No Hit
AAACCAGAACCCATTCTGGAATACCGGCTGCGGGCTACCGGTGTCAGAAG70.11488593467914No Hit
ACTCACGTTTCCTTTAACCACATAATTAGAATCATTCTTGATGTCTCTGG70.11488593467914No Hit
GACATCACAACCCTTTTCTGAAGTAGTGCTTTTAGACAATACAAAGTAGC70.11488593467914No Hit
CACCGGGCGAGGGCGGGTCTCTCGGAGGAAGGACTTGAGGTCTCCCCCCG70.11488593467914No Hit
ACAAGTTAAGGCACACATCAGTGGAACTTCTGTGTGCCTT70.11488593467914No Hit
CAGGATGCAAGGGGCTTTCTGCCCACCGCAAAGCAACCCCCAGCCCCCCA70.11488593467914No Hit
GGGGATGCCGGGGTCACTGAGCTTGATGAATGGGCCACACTCACTGTCGA70.11488593467914No Hit
CAGCAATCCACTTGTCTAGACCCTGTCCATCCTTCAAGTCCAAACTCTAA70.11488593467914No Hit
ACTGCTGTTTTTTCACTAGATTGTGTGAAGATCATAAACTGACGAACTTTATCCTTCTGCGATGATTTC70.11488593467914No Hit
ACTGTTGTTGGCTGTTGGTGAGGAAGGTGAAGCACGCAGTTGCCTTCTCG70.11488593467914No Hit
CTGCCCCCATAGAGCGCTCCCCGCACCGGCCCATCCTGCAGGCGGGGCTG70.11488593467914No Hit
CAGGGCACTGGCCAGCTGTTTGGCAACTTTGAATTTCCATGGTGTGGTAAGGAC70.11488593467914No Hit
GGGCAGACTTGGAGGAGAAGGACCAGGAGGATGTGGAGTGCCGAAATCTCCAGTC70.11488593467914No Hit
ACATTGGTGGTCTTTTTGTAATAGTCTATATTGTTGATATCTCTGGCGAG70.11488593467914No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)