FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003447794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003447794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6093
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN189031.0192023633678No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACACAGGCGCATCTCGTATGC2083.413753487608731No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACAGGCGCATCTCGTAT671.099622517643197No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACAGGCGCATCTCGTATGCC440.7221401608403085No Hit
TCTTATACACATCTCCGAGCCCACGAGACACAGGCGCATCTCGTATGCCG440.7221401608403085No Hit
CCGGAAAGTCCACGCTCACCATGTGTGACTTGATTAGCAGGTCAAAAGTG430.7057278844575743No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACACAGGCGCATCTCGTATG410.6729033316921057No Hit
CCTAAAAGAGTCATACACAGGCCCAGGGCAGTTCTTGGGTGGGTCCATCC390.6400787789266371No Hit
ACCTTAACATGATGTTATCTGTATTTACCTATAGTCTAAATTATACCATC340.5580173970129657No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGGCGCATCTCGTATGCCGT310.5087805678647629No Hit
GTTCCCTACAGATTGCGAGAGAGCTGCATCAGTTCACTTTTGACCTGCTA300.4923682914820286No Hit
ACCCCAGCAAAAGCAACCAGGAAGTTCTGGAGTTTGTCACCAGTGGAGGC290.47595601509929425No Hit
ACGAAATATTCTGGGTGGCACGGTCTTCAGAGAAGCCATTATCTGCAAAA270.4431314623338257No Hit
TTATACACATCTCCGAGCCCACGAGACACAGGCGCATCTCGTATGCCGTC240.3938946331856229No Hit
CGAGGTCATAGTTCCTGTTGGTGAAGCTAACGTTGAGGGGCATCGTCGCG230.37748235680288855No Hit
GAGGGGAGCCTCACGTTGGTCCACATCCTGCGGCCGCTCCTTGTAGCCAA230.37748235680288855No Hit
CCTCACTCCAGGTCAGTGAGCCCACCTGACTTGGTGCAGGCTCCCAGCAA220.36107008042015426No Hit
TTCATGGTCCATTCGTGTAGTTGAACCCTAAAAATTAAGCACATTATAGA200.32824552765468573No Hit
AGCAGCCAGAAATGTTTTGGTAACAGAAAACAATGTGATGAAAATAGCAG200.32824552765468573No Hit
CCACGGCCGGCCCTCTAGGGACTCGAACAGAGACCTCTGCAGCTGTGGGT180.29542097488921715No Hit
AACAAGGGGTGCCGCAGCCCCAGATCTAGCCCCAAGTGAGACCTGAGGGC180.29542097488921715No Hit
ATGCATATTAAAACAAGATTTACCTCTATTGTTGGATCATATTCGTCCAC180.29542097488921715No Hit
GAGCCTAGGAACCCCTTGTTACCCACCCCCCAGCTCCCCAGCCCTCAGGT170.27900869850648286No Hit
CACCCCTGCGCTAACAGGTTTCTGTCCAGGCAGTGCACCCAGCAACCCCC170.27900869850648286No Hit
CCCCATAAGCATGACGACCTATGATGATAGGTTTTACCCATCCACTCACA160.26259642212374856No Hit
ACAGAAGCGGGCAAAGGGGCGACCGGACCCGAGCCCAGGGAGGCGTACAT160.26259642212374856No Hit
GCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCC160.26259642212374856No Hit
GTGCGTGGCTCTCACCACCCGCACGTCTGTAGGGGAGTACATCAAGACCT160.26259642212374856No Hit
GGTCCTGTGTGAAATTATGTTTGTTCCAACTTACTCAATTGAACTAATCA160.26259642212374856No Hit
CACATCTCCGAGCCCACGAGACACAGGCGCATCTCGTATGCCGTCTTCTG160.26259642212374856TruSeq Adapter, Index 18 (95% over 24bp)
GCAAAGGCCAAAGATAAAATGCTTACATACAGTTCTGTTTCT150.2461841457410143No Hit
CCCAGGATAGGACATTGATGCCAATTCTTACCTAAGACTTAATGAGGCTT150.2461841457410143No Hit
AAACTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGTGCCTTGACGAT150.2461841457410143No Hit
TAATCCACTTCGATTTGGAAATGGAATTTGAAGAAAGGCAAGCCTATTTCTGAGTGCCTGCAACTGT140.22977186935828No Hit
GAATATAGTTTTCTCTCCTGAATTGGAAAGAAGCCTTCAAAAGCAACATA130.2133595929755457No Hit
CACTAAGTGCGTCTCCGAGATAGCAGGGGACTGTCCAAAGGGGGTGAAAG130.2133595929755457No Hit
ACAGTTGCAGGCACTCAGAAATAGGCTTGCCTTTCTTCAAATTCCATTTCCAAATCGAAGTGGATTA130.2133595929755457No Hit
GCAGCGCAGTCTGAGGAATAAAATTGGAGAAAGTTGGTGGCTAAACCGGG130.2133595929755457No Hit
CGATCTGCCATGTGTGCATTCCCTATCAAATATGTCAACGACTTCTTCAA130.2133595929755457No Hit
ATTCATCTACAAAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTC120.19694731659281145No Hit
CAAAAAGGGATTCAATTGCCATCCATTTAACTGGAATCCGACCCTAAAGA120.19694731659281145No Hit
AGAAACAGAACTGTATGTAAGCATTTTATCTTTGGCCTTTGC120.19694731659281145No Hit
CCCCTCCCCAGAGGGCAGAGCCCCCCAAGCCTACTTCAGAGCTCCCTTCT120.19694731659281145No Hit
ACTGCTGTTTTTTCACTAGATTGTGTGAAGATCATAAACTGACGAACTTTATCCTTCTGCGATGATTTC120.19694731659281145No Hit
GGAACATTCTCCAACACTTCCACATGCAATTCTTCTCCAGTAAGCCAGGA120.19694731659281145No Hit
CATATCAGCTTTAGGAAAGATGATGAAAGAAAAGAAATGGGCCCAGAGGG120.19694731659281145No Hit
AACAAACCTAGAAGCCTTAAGAAATTCATAAATTTCTAGTATTGATACCA110.18053504021007713No Hit
AAAGGGACCCACAGACTGGAACAGCCTCACATTTCGGCTTTAGAACAAAT110.18053504021007713No Hit
CAAAGCAGTGGATCCACTTCCTCCGCTCCGACCGCTGGCCCCCCACATCC110.18053504021007713No Hit
ACACCTTGCAGTGGAACTCCACGTCGCTGCCCAGCACCGCCGTCTGGTTG110.18053504021007713No Hit
TATGAGATGCTGGTTTTCTACCCAACGGCCCTGCAGCCAGCTCACGTCCA110.18053504021007713No Hit
GAACTGAAGATAATGACTCACCTGGGGCCACATTTGAACATTGTAAACTT110.18053504021007713No Hit
GAGCCTAGGAATCCCTTGTTACCCACTCCTCAGCTCCCCAGCCCTCAGGT110.18053504021007713No Hit
TCTCCAGCCAGGAAAGTGGGGGGAAATCACAAAGACTTCATGGAAAAGTG110.18053504021007713No Hit
AGGGCAGTCTCTGCTAAGGACGCCTCTTCCTGTGGTATTGGGTGGTGTTC110.18053504021007713No Hit
CTGTCTCTTATAACATCTCCGAGCCCACGAGACACAGGCGCATCTCGTAT100.16412276382734287No Hit
ACTCACGTTTCCTTTAACCACATAATTAGAATCATTCTTGATGTCTCTGG100.16412276382734287No Hit
AAAGCGGCTGGGTGGTCTTGGTGGGGGAATAAAGGGAGCACCCTTTCACC100.16412276382734287No Hit
CAACACAGTGGAGCGAATTCCTTTGGAAAACCTGCAGATCATCAGAGGAA100.16412276382734287No Hit
CCACGGCCGACCAGCTGGAGATGGTGACCGAGCTGCTGGGAGGAGACATG100.16412276382734287No Hit
AAAACATGGTAAGAAACCATTCTTTTCCCCCATTCTTTCTGTAAACAGTT100.16412276382734287No Hit
TTGCAGGTGGTCTTTCGGGGTGCTCCTGTGGGAGATCTTCACTCTGGGCG100.16412276382734287No Hit
CACCAGAATTTATTTTTAATTGTCATACTAATTTGGTGATCAGACCCTCT100.16412276382734287No Hit
GGGAAAAGTACCAGACACAAATGTTTATGTTGGAGAATCTTACAAGATGT100.16412276382734287No Hit
ACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATGGAATCCCGTAACTCTT100.16412276382734287No Hit
TGCTGATTGGTTTCGTAATCGTAGCTGGCATGATGTGCATTATTGTGATG100.16412276382734287No Hit
ACTGTTGTTGGCTGTTGGTGAGGAAGGTGAAGCACTCAGTTGCCTTCTCG100.16412276382734287No Hit
ACATTGGTGGTCTTTTTGTAATAGTCTATATTGTTGATATCTCTGGCGAG100.16412276382734287No Hit
TATACACATCTCCGAGCCCACGAGACACAGGCGCATCTCGTATGCCGTCT90.14771048744460857No Hit
ACACCGTGCCGAACGCACCGGAGCCCAGCATTTTGATCTTTTTGAATTCA90.14771048744460857No Hit
CAGGATGCAAGGGGCTTTCTGCCCACCGCAAAGCAACCCCCAGCCCCCCA90.14771048744460857No Hit
GATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGT90.14771048744460857No Hit
AACAGGGTGTTGATCTTAGAGTCCAACAAATTATGCCTAAAAAAAAAAAA90.14771048744460857No Hit
ACATTGGTGGTCTTTTTGTAATAGTTTATATTGTTGATATCTCTGGCGAG90.14771048744460857No Hit
CCAGGAAATAGAAAACTACAGGACGTTATCCAGGAAATAGAAAACTAC90.14771048744460857No Hit
CAGGGCACTGGCCAGCTGTTTGGCAACTTTGAATTTCCATGGTGTGGTAAGGAC90.14771048744460857No Hit
GAGTACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACT90.14771048744460857No Hit
CCACGGCCGACCAGCTTCTCTGTTTTATATTTTTTTCATCATCTCAGCCC80.13129821106187428No Hit
GGGTCCGTAAAAATGCTGGAGACATCTCACATTGTTTTTGTTGACGATCT80.13129821106187428No Hit
CCATGCTGCTCGGGATCCATATGTGGTAATCATTATTTAATGGAAACTCT80.13129821106187428No Hit
CTGCCCCCACAGAGCGCTCCCCGCACCGGCCCATCCTGCAGGCGGGGCTG80.13129821106187428No Hit
GGGGCGCCAGGTGGCTGAGTCCTGGCGCTGTGTCCTTTCAGGATGGTGGA80.13129821106187428No Hit
AAGAAGAAAGAATTCAGAGAAAAAAAGATATCACATACTCTTACCATTCG80.13129821106187428No Hit
GATGAAGATCGCAGACTTCGGGCTGGCCCAGGACGTGCACAACCTCGACT80.13129821106187428No Hit
CTCAACAGGCAAAACGGGAGGAGGAGCGCAGCCCAGCTGCCCCACCACCT80.13129821106187428No Hit
GTAGTTTTCTATTTCCTGGATAACGTCCTGTAGTTTTCTATTTCCTGG80.13129821106187428No Hit
CATCTCCGAGCCCACGAGACACAGGCGCATCTCGTATGCCGTCTTCTGCT80.13129821106187428TruSeq Adapter, Index 18 (96% over 26bp)
GATGAGCAGCAGCGAAAGCGCCTTGAGGCCTTTCTTACCCAGAAGCAGAA80.13129821106187428No Hit
GGGGATGCCGGGGTCACTGAGCTTGATGAATGGGCCACACTCACTGTCGA80.13129821106187428No Hit
GCAGCAAATTCAACCACCAGAACATTGTTCGCTGCATTGGGGTGAGCCTG80.13129821106187428No Hit
ACAGTTGCAGGCACTCAGAAATAGGCTTGCCTTTCTTCAAATTCCATTTC80.13129821106187428No Hit
GAGTGGGGTTGGTAGGCATTGAGAGGTGGATTGGGACCTTTGTAGTAGAA80.13129821106187428No Hit
ACTGTTGTTGGCTGTTGGTGAGGAAGGTGAAGCACGCAGTTGCCTTCTCG80.13129821106187428No Hit
TTCACAGTATCTTTTGTGAAATAGTCAGAAGTTGGTCAGAATTTCACATT80.13129821106187428No Hit
ATGGGGCCGTAGGAACCGGCTCCGGGGCCCCGATAACGGGCCGCCCCCAC80.13129821106187428No Hit
GGGCAGACACCTATCACTTTCGGGGCTGGTAGACTTTCTATTGAAGCAAT80.13129821106187428No Hit
GCAGTTACTGGACTTGTCCATGCGGTGACCCTCCTTCAGCAGCTTGAAAA80.13129821106187428No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACACAGGCGCATATCGTATGC70.11488593467914No Hit
GGTGCTAGTGGGAAACAAGTGTGATTTGCCAACAAGGACAGTTGATACAA70.11488593467914No Hit
GCACCAAAAGTTATAATTTTTCAATTACTTAATGTTTACATCAGATCATT70.11488593467914No Hit
AGAATATGAAGAAGAACACAAACCAAGTAGCTGTGGGTTGAACCTGGACG70.11488593467914No Hit
CACCACATTACATACTTACCATGCCACTTTCCCTTGTAGACTGTTCCAAA70.11488593467914No Hit
GACTCCCGGCCGGTCGGACATTCCTGCTTTATTGTGTTAATTGCTCTCTG70.11488593467914No Hit
GCAGGAGGATAAAGACCTGGTCCATGGAAATGTGTGTACTAAAAACCTCC70.11488593467914No Hit
TAGATAAGACTGCTAAGGCATAGGAATTTTCGTAGTACATATTTCCTCTG70.11488593467914No Hit
AAGGCACACAGAAGTTCCACTGATGTGTGCCTTAACTTGT70.11488593467914No Hit
CACAGAGACTTGGCAGCCAGAAATATCCTCCTTACTCATGGTCGGATCAC70.11488593467914No Hit
GGGAAGGGACTGCACTTACCTCACGCCAGCCCGGGGTGCTGTGGGGGCGG70.11488593467914No Hit
GCTTTTGCCTTCCATTCTCCCTGTAAGGGTCCACGTGAACAGCAAGTTTG70.11488593467914No Hit
GCACAGCTTCTCGGCCGTCAGGGGGATGGTCTCCTTCATCTTAGAGGCCA70.11488593467914No Hit
CCAGAAATAGAAAACTACAGGACGTTATCCAGAAATAGAAAACTAC70.11488593467914No Hit
GACTGGAGATTTCGGCACTCCACATCCTCCTGGTCCTTCTCCTCCAAGTCTGCCC70.11488593467914No Hit
AAAGCAGAATTCTGATTTCTGCAATGTAGGCCACCTTAGAGCAAAATACA70.11488593467914No Hit
AACAAGGGGTGCCACAGCCCCAGATCTAGCCCCAAGTGAGACCTGAGGGC70.11488593467914No Hit
CAGAAGGCTGCAGTCAACTCACATATATGCCCTTTGTTTCTGACATAGAA70.11488593467914No Hit
TCTCCCTCTTATTGTTCCCTACAGATTGCGAGAGAGCTGCATCAGTTCAC70.11488593467914No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)