FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003447872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003447872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTACCACACCATGGAAATTCAAAGTTGCCAAACAGCTGGCCAGTGC32.142857142857143No Hit
ACAACGATGTGGGCAAGACTTCTGCCTATTTTAACTTTGCATTTAAAGGT32.142857142857143No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21.4285714285714286No Hit
CCCAGGATAGGACATTGATGCCAATTCTTACCTAAGACTTAATGAGGCTT21.4285714285714286No Hit
GGGTCCGTAAAAATGCTGGAGACATCTCACATTGTTTTTGTTGACGATCT21.4285714285714286No Hit
CAAAGGGGCCATCAGGGACCACCTCGAAACACAACTTGCTTTACTGCACG10.7142857142857143No Hit
CTCCCATGACTCCTGCCGGGCAGGTCTTGACGCAGTTGCGGCCGTCAATG10.7142857142857143No Hit
CTCCCCATTTCTCTTTTGTCCCCCTCTGTCACCGCATATGACCATCACTT10.7142857142857143No Hit
CGAGGTCATAGTTCCTGTTGGTGAAGCTAACGTTGAGGGGCATCGTCGCG10.7142857142857143No Hit
GGCTATTTGCCTTTTGAGTATTCTTTCTACAAAAGGAAGTAAATTAAATT10.7142857142857143No Hit
GACCTGAAGATAATGACTCACCTGGGGCCCCCTTTGCAGCTTGCAAGGTT10.7142857142857143No Hit
GCAGTGTCCGGTTTATGAGCTGCTGGTTTTCTTTTTTATGGTCCTGCAGC10.7142857142857143No Hit
CCCTTGGATCAAATGGGTGAACAGAATATTAAGTTGAGTGTCCTCTT10.7142857142857143No Hit
AACCACAGTGGCCAGGGCCCTGCATACTGCTCTATTTCTTTCCCCAGTCT10.7142857142857143No Hit
GGGAGAGTTGGGAACATACTTCTATTTATATTCATAAGTATAGGAAGAAA10.7142857142857143No Hit
CCACTTGCTGGGAGGGTATTATCTCTGGGGGACAAGGGCAAGACGCCCAG10.7142857142857143No Hit
TCTTTATAATGCACCTGCCTTTGTTCCTCATAATTACATTCATGTTCATT10.7142857142857143No Hit
AGTATATTCACATGTCGTGTTTTCCTGTACAAAGAAATGTGACAGTAATC10.7142857142857143No Hit
CACCAGTTACCTGTCCTGGCCATTTATAGAAACCGAGGTATGAAATTCGC10.7142857142857143No Hit
CCAGCCACGTGAGGCCCTGCAATAGAAAACGCTTTTTCCTTTGGGACAAT10.7142857142857143No Hit
TGAACCCAAGACTCCCCTATAACCTAGGCCGTACGAACTATGACTACCTG10.7142857142857143No Hit
TCAACAGAAAGAAATGGATTTATTTGCTCTTCGCGTTTAATTAGTACAAA10.7142857142857143No Hit
GGTCTCTCATGGCACTGTACTCTTCTTGTCCAGCTGTATCCAGTATGTCC10.7142857142857143No Hit
CACCACATTACATACTTACCATGCCACTTTCCCTTGTAGACTGTTCCAAA10.7142857142857143No Hit
CCTGGGAGGACTCACTGATTAGTTCTATTGTGTTCATTTGTCTGTGTCTT10.7142857142857143No Hit
GCCAGAGACATCAAGAATGATTCTAATTATGTGGTTAAAGGAAACGTGAGTACCCATTCTCTGCTTG10.7142857142857143No Hit
CCCGCCCGCATTGTCCACGTTCTTTTTGTCCTTCTCCTCTATGTTTGTCC10.7142857142857143No Hit
CTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGACAT10.7142857142857143No Hit
CCTTCTCTTACTTTGTTTTCTACATACCCCACTCCCCTTCCTGTGAGGAG10.7142857142857143No Hit
GAGCCGCAGCCTCGCCACTCGTCCATGGATCCCTACCGAACTGGCTGTTG10.7142857142857143No Hit
CCAGGGTCTTTCTGTCACACATCTAACAAACAGCCAGTGCTAGAAAGGGGTTTATCCTCGAACGCAGAGAGC10.7142857142857143No Hit
AAACGGGAATGCGTATTTTTCCGAGTTGCTTACTTCCTAGTGCGGGTTAA10.7142857142857143No Hit
GAGGAAGGAGGGTGGGCACACTAACCTGCAGGAAGGAGAGGTCGGCATTG10.7142857142857143No Hit
ACTTCTGCAGTCCTGGCAAGGGAAACAATCTGCGTGTTGCTCCACTGTCC10.7142857142857143No Hit
CACCAGTTACCTGTCCTGGTCATTTATAGAAACCGAGGTATGAAATTCGC10.7142857142857143No Hit
GCTTCCGGCTTTGCGCTCCCCGCTCCAGAAGTCTCCCGCGACGACGCCCC10.7142857142857143No Hit
ATTTCACACTCCTAAGTTGATCTTTAATAGATACTGTCCCCGTGTGGGTT10.7142857142857143No Hit
CCCTTGGATCAAATGGGTGAACAGAATATTAAGTTGAGTGTCCTCTTCTA10.7142857142857143No Hit
ACACTTGCAGGCACTCCGATCTAGTCTTGCCTTTCTACACCTTCCTCTTC10.7142857142857143No Hit
GGACCAGAGATCCGGGCCCCCGCTTGGTCATTGAGCATAGGGGAGCCCAG10.7142857142857143No Hit
GGACCGCGTAGCTGACCCTGCCTACCTGCCTACGCAACAAGATGTGCTTA10.7142857142857143No Hit
GAGCCTAGGAACTCCTTGTTACCCACCCCCCAGCTCCCTAGCCCTCAGGT10.7142857142857143No Hit
CAACATATCTAACTTGACAAACTTCCTGTCGGCTGCGTGGGTGGCGGGCA10.7142857142857143No Hit
AATGTTTGGAGACCAGTATAAGAGGAAGCGGGATGCCGGGGTGACCTGGC10.7142857142857143No Hit
GCTACCCAAGCGCCATATCCTAAAGGACCTGAGCCCTTCACCTTTCTGCA10.7142857142857143No Hit
CCACATATGCGACACCTGTGGCCATGTGGAGGGGTGGCTGTATCTTTGCA10.7142857142857143No Hit
GAGACAGTCGCCCCTATGCGCATGGTGAGATTTTTTTCCAGCAGAGATTG10.7142857142857143No Hit
GCGTAAGCCGGGCTGAAAGCCGGGGCCTACTGCCCTGCCCCCCCACTCCT10.7142857142857143No Hit
AAGTTCCAGCAGATGCCACATAAGGGGTTTGCCATTTGATACCCCTGGGG10.7142857142857143No Hit
CAGTAGGCTTGTTCTAGCCTGAAATTGCAGCCACACCCTAGGCAATGGTT10.7142857142857143No Hit
CCTCACTCCAGGTCAGTGAGCCCACCTGACTTGGTGCAGGCTCCCAGCAA10.7142857142857143No Hit
CAAAAATGGTTTTTCATGCACTTCTTTAAAAAGTTCAACAGCAGCTGACA10.7142857142857143No Hit
CAACGTGAGATAAACCTAGATAGTTGGGGCTTTTCTTCCTATACTTATGA10.7142857142857143No Hit
CTCCCATGACTCCTGCCGGGCAGGTCTTGACGCAGTGGGGGCCGTCAATG10.7142857142857143No Hit
CCCCATAAGCATGACGACCTATTATGATAGGTATTACCCATCCACTCACC10.7142857142857143No Hit
CTTCCCCGACTCATGTTGATCGATTCCCCCACAACCTACGGCATCTCATA10.7142857142857143No Hit
CTACGGACTTGTGGGGGTGTCTCGGACATACTTCTTTTTGTATTCATAAG10.7142857142857143No Hit
AAATACAGGATTATGTACATAAATGAGATCAAAGTATTTGGAAAGGATCC10.7142857142857143No Hit
CCAACCTAATCGTGGTCCAGAGAGGTCACAGCCCACCCACATCATCCTTG10.7142857142857143No Hit
AACAATGAATGACCAACTGGAAAACTCATATTTAATCTGAAATATTAAAA10.7142857142857143No Hit
GTCTCGCAGTCTCGTGCTCACGGAGCGTTTAAGAGCCAGGGTGCTGCGTG10.7142857142857143No Hit
AAAATTCACGCCCCGGTCCAGCTAGTCTATATTGCCTCCGACGTCAGTTC10.7142857142857143No Hit
GAACCCACATCGATCAGGAAATGGAATTTGAAGATTCGTAATTCCATCTC10.7142857142857143No Hit
CCTTGGGAGATGTCAGATTGAATTTGCAAAGGCATATTAGGAACTCTGTG10.7142857142857143No Hit
CCAGCCACCATGATGTGCATCATTCATTTGTTTCATGAAATACTCCAAAG10.7142857142857143No Hit
GTTTTTTTTTATAAGTGGCTAGATGCTTTTGTATTGCTATTTTCCAAATTTTTCCCTT10.7142857142857143No Hit
CATGGGAAAGTATAATTTTAGGGACTGAGGTGTAGGATCTTCGATGCAAG10.7142857142857143No Hit
CCCCCCACACCTCGTCCTCCGCAACATTATAACCTTTCCTCGGCGTGAAA10.7142857142857143No Hit
GCAGACAAACTCTACGTCTCCTCCGACCACTGTGGAGGCATTTGCCGGCA10.7142857142857143No Hit
GCTGCCCTCTCCTGCCTCGTCGCCTGTTCCTGCGCTACAGTAGGCAGCCC10.7142857142857143No Hit
CGCCACCTTGGGCTACCTGCCCACCCAACAGGACGTGCTGCGGGTCCGCG10.7142857142857143No Hit
AAAAAATATCTAATTTATGACAATAAAAACCTTACTTTATTTGGATTTGA10.7142857142857143No Hit
CAAACCTACTGGCTAAATTTTCAGAGTATAAGTTAGAAGAGGACACTCAA10.7142857142857143No Hit
GTCATGATAATGCTAGTAATGAATCTGATCTCCCTTCCTATTCTAAAAAC10.7142857142857143No Hit
GCCTCTGTCGTCATGCAATCTCATATGTCTCCTTGCCACCGGACTGCCCG10.7142857142857143No Hit
CCACGGCCGGCCCGTTAGCTCCGCAAACTGAGAGCTACGGAGGTGAGTGC10.7142857142857143No Hit
TGTGGGCTACAAGAACTACCGATACCGTGCGGGCTTCGTGCTGGCCCCAA10.7142857142857143No Hit
GGATCACCAGTGCTTTCATGCACTCTCTAATATAGCACAGCGTACCCTAA10.7142857142857143No Hit
GCAGCGCAGTCTGAAGAATAAAATTGGAGAAAGTTGGTGGCTAAACCGGG10.7142857142857143No Hit
GAACAGTGACAGGAGAATATTAGATTCCATAAATAAAAACCCTTATTAAA10.7142857142857143No Hit
TTGAATGCCATTAAAAATATCTAATGTATGACAATAAAAACCTTACTTTA10.7142857142857143No Hit
GCAGATTTCTAGAGGCCTAAATCCTCCACCCTTGTTCTCAAGAGGGTCAT10.7142857142857143No Hit
GCTGTCCACAGGCAGGGTGGTGCTGACCATGTGGACATTAGGTGTGGATG10.7142857142857143No Hit
GCAGGTTGACAGCTTCCAACTGGCTGAAGGTGGGTTTGATTTCAATGAAA10.7142857142857143No Hit
CAAAGCGAGCCTGGCCGGCCTCGCGCCTGTCGGGGAGTCGAGGCAGGGCC10.7142857142857143No Hit
GAGCCAGTGGCTGAAATTGGTGTCGGTGCCTATGGGACAGAGTACAAGGC10.7142857142857143No Hit
TTCTACAGTATTGATACCTTGTATGTGTACACAACTCATGTTGTAAGTAA10.7142857142857143No Hit
GGCCCCATGTCATGCCTATAAAAGATAGAAGTAAGAAGATCCCCTCCCTC10.7142857142857143No Hit
TTCCCTCTGGTCCTGTCAAAATTCGCTGTTCTGCCATTTCGCAAGCTCCG10.7142857142857143No Hit
ATCCTCCCGTCCCTTGTTGGATCTTTGCCTCTATGATTCCCCAGTCTCCT10.7142857142857143No Hit
GAGTACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACT10.7142857142857143No Hit
ACGAAATATTCTGGGTGGCACGGTCTTCAGAGAAGCCATTATCTGCAAAA10.7142857142857143No Hit
GGATAAACAACTCAATCCATCCTCTTAATATCCTCTTCAAACCTGATTTC10.7142857142857143No Hit
CCTAGAGATTGAATAGATCTTGAACTTTTTTTTTCTCCAGGGGGGAAAAA10.7142857142857143No Hit
GTACGCCCTTTTGCTGCTGCAGGCGTTGGCCCTGGCGCCGCTGGGCTCCC10.7142857142857143No Hit
AAACATCCCCTTAAATTGGATTAAAAAGAAATATACCTTTGTTGTTACCT10.7142857142857143No Hit
GTCAATGGTATTTACAGAAATGTTCCCCAGGGGTATCAAATGGCAAACCC10.7142857142857143No Hit
GGCAAGGACCCCATGACAAGTGCCCCCTCTCCCATCTTCCACAGAGCGGT10.7142857142857143No Hit
GTGCAGTTGGCGGGCTTGTCCATGCGGTGGCCTTCCTTCAGCAGCTTGAA10.7142857142857143No Hit
ATTATCGTGCATGTGGGATGTATTTGAAGCACCTGAATAGGCAAGTCGAG10.7142857142857143No Hit
ATGGTCATTTCAGTTTTCCTGTAGCAAAACCAGAAATCCTGACTTACGAC10.7142857142857143No Hit
ACAAATTACCATTAATGACTTCTTTGCCCAAGTGCAAGAACCCTTGATGG10.7142857142857143No Hit
GGACCCCCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGG10.7142857142857143No Hit
CATCCGGGCTCTTCAGCGTGGATTGGTAGAACCGACACCCCTTCTGGTGC10.7142857142857143No Hit
GGCAAGGTCCCCATGACAAGTGCCTCCTCTCCCATCTTCCACAGAGCGGT10.7142857142857143No Hit
AAGCATCAGAGGGGAATTTGGCATTGCCTACAGCATTTAGTTTTCTTTCC10.7142857142857143No Hit
GCTTGGGAATGCGCTTTGCTTTGGGTGTGTCCAAAGCCTCATTAAGTCTT10.7142857142857143No Hit
ATACACTACGAACCTCTCCCCCGTCTTTTCTCTACACCAGACCCCTTCCT10.7142857142857143No Hit
GTGCAGTTGGCGGGCTCGCCCATGCGGTGGCCCTCCTTCAGCAGCTTGAA10.7142857142857143No Hit
GAAATCATCGCAGAAGGATAAAGTTCGTCAGTTTATGATCTTCATACAATCTAGTGAAAAAACAGCAGT10.7142857142857143No Hit
GTTCCTCTTTGCCTCTGCTTGTTTTTCAGTGTCCGCTTTTTCCCACATTT10.7142857142857143No Hit
GGGAAGGGACTGCACTTACCTCACGCCAGCCCGGGGTGCTGTGGGGGCGG10.7142857142857143No Hit
GCTTTTGCCTTCCATTCTCCCTGTAAGGGTCCACGTGAACAGCAAGTTTG10.7142857142857143No Hit
GTGCAGTTGGCGGGCTTGTCCATGCGGTGGCCCTCCTTCAGCAGCTTGAA10.7142857142857143No Hit
GTAAGAATTGCTAAAGTTTGTCGACATTTAATGTTTACTGTCACATTTCC10.7142857142857143No Hit
GAGACCAAATTTGAGATGATCAAACTTATAGATATTGCACGACAGACTGC10.7142857142857143No Hit
CCTGTTTATGGCTTAAGTGCGCAGAATATTGCCTTGGGCATCGTCCTCTA10.7142857142857143No Hit
CCTTTAAGTGCACGATCATAATCTATTACATTATCAGCAAGCCATAAGCT10.7142857142857143No Hit
CTACTTCCATCTTGTCAGGAGGACAGGCCCTGACACAGGATGTTTGATCC10.7142857142857143No Hit
AAGATAATTTTAACTAGCTGTTTTTCCTTTTATTTTTCTCTTTTAGCATC10.7142857142857143No Hit
TCTCCCCAACCTTCCTTGGTCTCGGGTTTTTATGGCGCAGCTTATGCTTT10.7142857142857143No Hit
TTGATTGTCCTGCACCTATGTCTCTTCCCCCCGACATGCGGTTGCGCCGG10.7142857142857143No Hit
GCTTGATCTGAAGGGGACCGGAAAGCTGAACAATTGTGGGATTTGTTCTA10.7142857142857143No Hit
GGAACATTCTCCAACACTTCCACATGCAATTCTTCTCCAGTAAGCCAGGA10.7142857142857143No Hit
GGGTTTCCTTTTTATCAGTTGGGTAGTGATAGGGAAGGAATCAACCTCAT10.7142857142857143No Hit
CCACATATGCAACACCTGTGGACATGTGGAGTGTTGGCTGTATCTTTGCA10.7142857142857143No Hit
CACACACAAACTCAGCATCTGCAGAATGAAAAACACTCAAAGGATTAGAA10.7142857142857143No Hit
GGAACAGAAAGAAATGGATTTATCTGCTCTTCGCGTTGAAGAAATACAAA10.7142857142857143No Hit
AGCTCAACAATTATGCTGTCCACAGGCAGGGGGGTGTCGACTAAGGGGAT10.7142857142857143No Hit
GAACACCACACCGCCATTGCCAGCCCCCAGCTCACTGATCTTCTCAAAGT10.7142857142857143No Hit
ACTGCTGTTTTTTCACTAGATTGTGTGAAGATCATAAACTGACGAACTTTATCCTTCTGCGATGATTTC10.7142857142857143No Hit
GAGTGGGGATGTTAGTCGTTGAGCGGTGGTTTGGGGCCTGTGTCGTAGAC10.7142857142857143No Hit
GTCATGATAATGCTAGTAATGAATCTGATCTCCCTTCCTATTCTAAAAAT10.7142857142857143No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)