FastQCFastQC Report
Wed 1 May 2024
EGAF00003447982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003447982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40312351
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG4787661.187640978815649Illumina Paired End PCR Primer 2 (100% over 31bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG2736630.6788564626260573Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATAGCGATCTCGT850810.21105442349417924Illumina Paired End Sequencing Primer 2 (100% over 36bp)
CAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACC680790.16887876373174068Illumina Paired End PCR Primer 2 (100% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)