FastQCFastQC Report
Wed 14 Sep 2022
EGAF00003447982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003447982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40312351
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGA4776841.1849569378873488Illumina Paired End PCR Primer 2 (100% over 32bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGC2732280.6777773888702249Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATAGCGATCTCGTA850340.21093783391596285Illumina Paired End PCR Primer 2 (97% over 36bp)
CAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCG680180.16872744534299178Illumina Paired End PCR Primer 2 (100% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)